低共熔溶剂法提取枣渣中黄酮类物质

Extraction of flavonoids from jujube residue by deep eutectic solvent method

目的 探究低共熔溶剂法提取枣渣黄酮的最佳提取工艺。方法 以冬枣枣渣中的黄酮提取率为评价指标,在低共熔溶剂的种类、摩尔比、料液比、超声时间、超声温度、超声功率等单因素实验基础上,采用中心组合(Box-Behnken, BBK)实验模型对提取工艺进行优化。结果 最佳提取条件为:以氯化胆碱-丙三醇-乙醇(摩尔比1:1:1)为提取剂,在料液比1:60 (g/mL)、超声功率105 W、超声温度70℃、超声时间32 min条件下,黄酮提取率为5.88%。结论 采用低共熔溶剂作为提取剂提取枣渣黄酮,提取率明显优于传统乙醇提取法。本研究结果为枣类资源的充分开发利用提供一定理论支撑。

Objective To study the optimum extraction process of flavonoids from jujube residue by deep eutectic solvent method. Methods The extraction rate of flavonoids from jujube residue was taken as the evaluation index, and the Box-Behnken(BBK) experimental model was used to optimize the extraction process on the basis of single factor experiments of deep eutectic solvent type, mole ratio, solid-liquid ratio, ultrasonic time, ultrasonic temperature and ultrasonic power. Results The optimal extraction conditions were as follows: Choline chloride-glycerol-ethanol(molar ratio 1:1:1) as the extraction agent, solid-liquid ratio 1:60(g/mL), ultrasonic power 105 W, ultrasonic temperature 70 ℃, ultrasonic time 32 min, the extraction yield of flavonoids was 5.88%.Conclusion Deep eutectic solvent is used to extract flavonoids from jujube residue, and the extraction rate is significantly better than that of traditional ethanol extraction method. The results of this study provide theoretical support for the full development and utilization of jujube resources.