松茸水解蛋白肽制备及其抗氧化活性的研究

Study on preparation and antioxidant activity of Tricholoma matsutake hydrolytic protein peptide

目的 制备松茸水解蛋白肽,并研究其抗氧化活性。方法 松茸蛋白经蛋白酶酶解,对酶的种类和组合进行优化并制备水解蛋白肽。通过测定1, 1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl, DPPH)自由基、2,2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸[2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), ABTS]自由基及羟自由基的清除率,研究其抗氧化活性。结果 优化后最佳水解酶组合为风味蛋白酶:碱性蛋白酶质量比为1:3,最佳水解工艺条件为底物质量浓度为4 mg/mL,作用时间6 h,酶添加量质量分数为2.0%,松茸水解蛋白肽水解度为33.48%±0.2%,可溶性氮含量为72.32%±0.15%,且具有较强的DPPH自由基和ABTS自由基清除能力。结论 在优化后的条件下,松茸蛋白酶解效果最佳,且松茸水解蛋白肽有一定的抗氧化活性。

Objective To prepare Tricholoma matsutake hydrolyzed protein peptide and study its antioxidant activity. Methods Tricholoma matsutake protein was hydrolyzed by protease, the types and combinations of enzymes were optimized and hydrolyzed protein peptides were prepared. Its antioxidant activity was investigated by measuring the clearance rates of 1,1-diphenyl-2-trinitrophenylzyl(DPPH) radical, 2,2’-biazo-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS) radical and hydroxyl radical. Results After optimization, the best combination of hydrolytic enzymes was that the mass ratio of flavourzyme to alkaline protease was 1:3, the optimal hydrolysis conditions were as follows:The substrate concentration was 4 mg/mL, the reaction time was 6 h, and the mass fraction of enzyme addition was 2.0%, the degree of hydrolysis of Tricholoma matsutake hydrolyzed protein peptide was 33.48%±0.2%, and the soluble nitrogen content was 72.32%±0.15%, it also had strong scavenging ability of DPPH free radical and ABTS free radical.Conclusion Under the optimized conditions, the enzymolysis effect of Tricholoma matsutake is the best, and the hydrolyzed protein peptide of Tricholoma matsutake has a certain antioxidant activity.