超高效液相色谱-串联质谱法检测水牛角特征肽

Identification of specific peptides from Bubali Cornu by ultra-performance liquid chromatography-tandem mass spectrometry

该文应用超高效液相色谱-串联质谱分析及数学集合理论寻找水牛角特征肽。首先,nano LC-LTQ-Obitrap-MS应用于水牛角、牦牛角、山羊角及猪蹄甲经胰蛋白酶酶切后的多肽组轮廓分析,其次,采用数学集合方法分析不同样品肽类组成的关系,当肽段仅存在于水牛角集合且不在其他集合中时,被认为是潜在的水牛角特征肽。进一步通过生物信息学比较确定了4个水牛角特征肽,以UPLC-QQQ-MS验证其专属性。结果表明4个特征肽可用于区分水牛角与山羊角、水牛角与猪蹄甲。该研究提供了一种寻找动物药特征肽的快速、简单的方法,可应用于动物药的质量评价,以保证质量可控及可追溯性。

This paper explored the specific peptides from Bubali Cornu by ultra-performance liquid chromatography-tandem mass spectrometry and based on mathematics set theory.Following the profile analysis of peptides from Bubali Cornu,Bovis Grunniens Cornu,Caprae Hircus Cornu,and Suis Cornu by nano LC-LTQ-Obitrap-MS after digestion with trypsin,the relationship of peptide composition among different samples was analyzed using the mathematics set theory.The ones that existed only in the Bubali Cornu set rather than in any other set were considered as the specific peptides of Bubali Cornu.The further bioinformatic analysis revealed four specific peptides from Bubali Cornu,whose specificity was verified by UPLC-QQQ-MS.The results showed that these four peptides could be used for distinguishing Bubali Cornu from Caprae Hircus Cornu and Suis Cornu.This study has provided a rapid and simple method for seeking the specific peptides in animal medicines,which can be utilized for quality evaluation of animal medicines,thus making them authenticable and traceable.