基于激光的G-四链体高分辨率熔解曲线分析

Laser-Based High Resolution Melting Analysis for Studying G-Quadruplux

结合所制备的高品质法布里-珀罗(F-P)光学微腔,利用基于激光的高分辨率熔解曲线(HRM)分析研究了G-四链体脱氧核糖核酸(DNA)结构的热稳定性。探究了K^(+)存在的情况下,诱导富含鸟嘌呤(G)的DNA单链形成G-四链体,不同K+浓度的DNA溶液,对应于溶液中G-四链体结构的不同热稳定性。实验结果表明,激光HRM曲线能很好地分辨出不同K^(+)浓度的DNA结构。此外,通过分析不同DNA溶液在任意温度下的激光阈值,发现利用较低的泵浦能量可以很好地降低曲线的熔解温度。

Combined with the high quality Fabry-Perot(F-P) optical microcavities,we studied the thermal stability of G-quadruplex DNA structure laser-based high resolution melting(HRM) analysis.DNA solutions with three different concentrations of K^(+),which corresponds to different thermostablility of the G-quadraplux DNA structure in the solution,are studied.Experimental results show that DNA structure with different K^(+) concentrations can be well distinguished by laser-based HRM curves.Further,by analyzing the laser thresholds of different DNA solutions at arbitrary temperature,it is found that the melting temperature of the curves can be well decreased by using a lower pump energy.