6种寒热属性中药短期及长期干预对小鼠机体脂质及能量代谢影响的对比研究

Comparative study on short and long-term intervention impacts of six Chinese herbs with cold or heat property on lipid and energy metabolism in mice

选择3种典型的寒性中药大黄、黄芩和黄连,以及3种典型的热性中药肉桂、干姜及附子,在小鼠中观察上述6种代表性寒热中药对动物机体代谢的调节作用,重点观察了短期(7 d)及长期(35 d)干预对动物脂质代谢及能量代谢的作用。采用real-time PCR法测定附睾脂肪组织和肝脏中脂质代谢基因mRNA表达水平;通过代谢系统检测机体耗氧量、二氧化碳生成量、热量消耗等。在短期干预下,热性中药显著降低脂肪组织指数,升高肝脏和附睾脂肪组织中乙酰辅酶A羧化酶(ACC)、脂蛋白酯酶(LPL)、肉碱-棕榈酰转移酶1(CPT-1)表达量;寒性中药促进附睾脂肪组织以上基因表达。在长期干预下,寒热中药对动物附睾脂肪指数均无明显影响,而寒性中药干预可以增加机体二氧化碳生成量和热量消耗,以及降低活动量。该研究表明短期寒热中药干预对动物机体代谢的影响明显强于长期干预,表现为短期干预寒性中药可促进附睾脂肪组织脂质代谢,热性中药可促进附睾脂肪组织和肝脏脂质代谢。长期寒热中药干预对机体脂质水平几乎没有明显影响,不过,长期寒性中药干预可促进机体能量消耗。该研究初步从机体脂质及能量代谢的角度观察了寒热属性中药对正常动物生理的影响,为基于生物体响应解释寒热属性中药的生物学基础提供参考。

This study selected three typical Chinese herbs with cold property(Rhei Radix et Rhizoma,Scutellariae Radix,and Coptidis Rhizoma)and another three with heat property(Cinnamomi Cortex,Zingiberris Rhizoma,and Aconiti Lateralis Radix Praeparata)to observe their regulatory effects on metabolism in animal organism,especially on lipid and energy metabolism in mice after a short-(7 d)and long-term(35 d)intervention.The mRNA expression levels of lipid metabolism genes in epididymal adipose tissue and liver were determined by real-time PCR.The oxygen consumption,carbon dioxide production,and energy consumption were detected by metabolic system.After the short-term intervention,the Chinese herbs with heat property significantly reduced epididymal adipose tissue index and elevated the expression levels of acetyl-CoA carboxylase(ACC),lipoprotein lipase(LPL),and carnitine-palmityl transferase 1(CPT-1)in liver and epididymal adipose tissues.However,those with cold property promoted the expression of above-mentioned genes in epididymal adipose tissue.After the long-term intervention,cold and heat Chinese herbs had no significant effect on epididymal adipose tissue index of animals,while cold Chinese herbs could increase carbon dioxide production and energy consumption and reduce activity.These findings demonstrated that the short-term intervention effects of cold and heat Chinese herbs on animal metabolism were significantly stronger than the long-term intervention effects.Specifically,the short-term intervention with cold Chinese herbs enhanced the lipid metabolism in epididymal adipose tissue,while the heat Chinese herbs promoted lipid metabolism in epididymal adipose tissue and liver.The long-term intervention with cold and heat Chinese herbs resulted in no obvious change in lipid level,but long-term intervention with cold Chinese herbs accelerated energy consumption of the body.This study preliminarily observed the effects of cold and heat Chinese herbs on normal animal physiology from lipid and energy metabolism,which would provide reference for explaining the biological basis of Chinese herbs with cold or heat property based on biological response.