紫外诱变法选育酒酒球菌酸-乙醇胁迫耐受菌株

Breeding of Acid-Ethanol Stress Tolerance Oenococcus Oeni by UV Mutagenesis

为选育优良抗性的酒酒球菌菌株,以酒酒球菌SD-2a为出发菌株,通过紫外诱变法获得酒酒球菌突变菌株,分别对突变菌株进行胁迫耐受性、β-葡萄糖苷酶活性及其在模拟酒中的苹果酸乳酸发酵速率和活细胞数量进行测定。结果表明,诱变产生的3株突变菌株UVae1、UVae2、UVae3,其胁迫耐受性优于出发菌株SD-2a,在模拟酒中UVae2最大细胞存活率可达到出发菌株的3.61倍,突变菌株的苹果酸乳酸发酵能力也较出发菌株有所提高,在培养到第8天时,突变菌株的苹果酸含量分别是出发菌株的86.0%、85.0%和84.9%,β-糖苷酶活力无显著变化。研究获得了3株胁迫耐受性提高的优良发酵菌株。

In order to breeding excellent stress tolerance wine making strains,Oenococcus oeni SD-2a was selected as starting strain,mutant strains were obtained by ultraviolet mutagenesis method,the stress tolerance,β-glucosidase activity,and malolactic fermentation rate of mutant strain and starting strain were tested in ATB and simulation wine medium,respectively.The results showed that,the three mutant strains UVae1,UVae2 and UVae3 show better tolerance to stress than the starting strain SD-2a,in the simulated wine,the maximum cell survival rate of UVae2 was 3.61 times that of the starter strain.And the malolactic fermentation rate of the mutant strain was also improved compared with the starter strain.On the 8th day of culture,the malic acid content of the mutant strain was 86.0%,85.0%and 84.9%of the starter strain,respectively,and theβ-glycoside activity didn’t change significantly.Three strains with improved stress tolerance were obtained.