摘要
Theranostic visualization of dextran at the nanoscale is beneficial for understanding the bioregulatory mechanisms of this molecule. In this study, we applied structured illumination microscopy(SIM) to capture the distribution of Cy5-Dextran at different incubation periods in living cells. The results showed that Cy5-Dextran could be absorbed by He La cells. In addition, we clarified that Cy5-Dextran exhibited differential organelle distribution(lysosomal or mitochondrial) in a time-dependent manner. Moreover,lysosomal Cy5-Dextran localization was found to be independent of the autophagy process, while Cy5-Dextran localized to the mitochondria triggered a pro-apoptotic event, upregulating the levels of reactive oxygen species(ROS) to accelerate mitochondrial fragmentation. This work uses a visualized strategy to reveal the anti-tumor bioactivity of dextran, which was achieved by regulating apoptosis and autophagy.
基金
supported by National Natural Science Foundation of China (Nos. 22107059, 21801158, 81870283, 82070382)
Program of Taishan Scholars Programme (No. 20190979)
Academic Promotion Programme of Shandong First Medical University (No.2019LJ003)
National Postdoctoral Program for Innovative Talents(No. BX2021123)
The China Postdoctoral Science Foundation (No.2021M691505)
the Jiangsu Postdoctoral Research Funding Program (No. 2021K125B)。