EXT2基因、KCNJ11基因单核苷酸多态性与血糖的交互作用对代谢综合征阴虚证易感性的影响

The effect of the interaction between the EXT2 gene,the KCNJ11 gene single nucleotide polymorphisms and blood glucose on the susceptibility to metabolic syndrome with Yin deficiency syndrome

目的探讨EXT2基因和KCNJ11基因的单核苷酸多态性(SNPs)与血糖交互作用对代谢综合征(MS)阴虚证易感性的影响。方法收集MS阴虚证、非阴虚证患者各108例,健康人235例,采用SNPscan;多重SNP分型技术检测SNPs位点。使用SHEsis对MS阴虚证、非阴虚证患者和健康者的基因进行分析。应用广义多因素降维结合逻辑回归模型检测SNPs与血糖交互作用对MS阴虚证的影响。结果携带KCNJ11基因rs5215-C等位基因多态性(CT+CC)个体中患MS阴虚证的风险是携带TT者的0.947倍(P>0.05)。携带EXT2基因rs11037909-C等位基因多态性(CT+CC)个体中患MS的风险是携带TT者的1.839倍(P<0.05)。由EXT2基因rs11037909位点和血糖这两个因素构成的模型是与MS阴虚证风险相关的最佳模型(P=0.001),携带CT/CC基因型的无高血糖个体患MS阴虚证的风险是携带TT基因型的无高血糖MS非阴虚证个体的4.216倍(P<0.05),携带TT基因型的高血糖个体患MS阴虚证的风险是携带相同基因型的无高血糖非阴虚证个体的3.737倍(P<0.05)。结论携带KCNJ11基因rs5215-C等位基因不增加MS阴虚证的易感性。而EXT2基因rs11037909-C等位基因是MS的危险因素,EXT2基因rs11037909与血糖交互作用会增加MS阴虚证的易感性,rs11037909-C等位基因与高血糖均是MS阴虚证的危险因素,二者交互作用后患MS阴虚证的风险比无危险因素者高。

Objective To investigate the effect of the interaction between single nucleotide polymorphisms of the EXT2 gene and the KCNJ11 gene and blood glucose on the susceptibility to metabolic syndrome with Yin deficiency syndrome.Methods Multiple single nucleotide polymorphism(SNP)typing techniques were used to detect SNPs in metabolic syndrome patients with Yin deficiency or non-yin deficiency syndrome and healthy people.The SHEsis software was used to analyze the genes of three groups of participants.A generalized multifactor dimensionality reduction combined with a logistic regression model was used to detect the effect of the interaction between the SNPs and blood glucose on the metabolic syndrome with Yin deficiency syndrome.Results Individuals with the KCNJ11 gene rs5215-C allelic polymorphism(CT+CC)had a risk of metabolic syndrome with Yin deficiency syndrome being 0.947 times higher than those with TT(P>0.05).Individuals with the EXT2 gene rs11037909-C allelic polymorphism(CT+CC)had a risk of metabolic syndrome being 1.839 times higher than those with TT(P<0.05).The model composed of the rs11037909 locus of the EXT2 gene and blood glucose was the best model related to the risk of metabolic syndrome with Yin deficiency syndrome(P=0.001).Individuals with the CT/CC genotype but without hyperglycemia had a 4.216 times higher risk of metabolic syndrome with Yin deficiency syndrome compared with those with the TT genotype but without hyperglycemia(P<0.05).Individuals with hyperglycemia and the TT genotype had a 3.737 times higher risk of metabolic syndrome with Yin deficiency syndrome compared with those with the same genotype but without hyperglycemia.(P<0.05).Conclusion Carrying the rs5215-C allele of the KCNJ11 gene did not increase the susceptibility to metabolic syndrome Yin deficiency syndrome.The rs11037909-C allele of the EXT2 gene was a risk factor for metabolic syndrome.The interaction between rs11037909 of the EXT2 gene and blood glucose will increase the susceptibility to metabolic syndrome with Yin deficiency syndrome.Both the rs11037909-C allele and hyperglycemia were risk factors for metabolic syndrome with Yin deficiency syndrome.After two risk factors interacted,the risk of developing metabolic syndrome with Yin deficiency syndrome was higher than those without risk factors.