PM2.5中16种多环芳烃的超高效液相色谱法和高效液相色谱法比较

Comparison between ultra performance liquid chromatography and high performance liquid chromatography in determination of 16 polycyclic aromatic hydrocarbons in PM2.5

目的比较测定大气PM2.5中16种多环芳烃(PAHs)的超高效液相色谱法(UPLC)和高效液相色谱法(HPLC),为PAHs测定方法选择提供参考依据。方法用玻纤滤膜采集大气中PM2.5,乙腈超声提取后,采用超高效液相色谱法和高效液相色谱法分析,分别使用ZORBAX Eclipse PAH、Waters PAH液相色谱柱分离,荧光检测器和二极管阵列检测器联用检测。结果UPLC法和HPLC法的分析时间分别为18 min和26 min,相关系数均>0.999,检出限分别为0.02 ng/m^(3)-0.18 ng/m^(3)和0.04 ng/m^(3)~0.26 ng/m^(3),定量限分别为0.06 ng/m^(3)~0.54 ng/m^(3)和0.12 ng/m^(3)~0.78 ng/m^(3),加标回收率分别为83.8%~110.3%和79.0%~120.2%,方法的相对标准偏差(RSD)分别为0.6%~1.3%和0.9%-5.2%。结论相比高效液相色谱法,超高效液相色谱法更节省时间,检出限和定量限更低,灵敏度更高,故在条件允许时,测定大气PM2.5中16种多环芳烃应优先采用超高效液相色谱法。

Objective To compare ultra-high performance liquid chromatography(UPLC)and high performance liquid chromatography(HPLC)for the determination of 16 polycyclic aromatic hydrocarbons(PAHs)in atmospheric PM2.5.Methods PM2.5 in the atmosphere was collected by glass fiber filter membrane.After ultrasonic extraction with acetonitrile,it was analyzed by UPLC and HPLC,and separated by ZORBAX Eclipse PAH and Waters PAH,and detected,by fluorescence detector and diode array detector,respectively.Results The analysis time of UPLC and HPLC were 16 min and 26 min respectively,the correlation coefficients were>0.999,the limits of detection(LOD)were within 0.02 ng/m^(3)-0.18 ng/m^(3) and 0.04 ng/m^(3)-0.26 ng/m^(3) respectively.The limits of quantitation(LOQ)were within 0.06 ng/m^(3)-0.54 ng/m^(3) and 0.12 ng/m^(3)-0.78 ng/m^(3) respectively.The recoveries were within 83.8%-110.3%and 79.0%-120.2%respectively,and the relative standard deviations(RSDs)of the method were within 0.6%-1.3%and 0.9%-5.2%respectively.Conclusion Compared with HPLC method,UPLC method saves more time,has lower LOD and LOQ and higher sensitivity.Therefore,UPLC method should be preferred for the determination of 16 polycyclic aromatic hydrocarbons in atmospheric PM2.5.