微波消解-电感耦合等离子体质谱快速测定葡萄籽提取物微量元素的研究

Study on Trace Elements in Grape Seed Extract by Microwave Digestion-inductively Coupled Plasma Mass Spectrometry

采用高压微波消解,建立葡萄籽提取物微量元素ICP-MS检测方法,并对葡萄籽中47种微量元素进行测定,应用芹菜成分分析标准物质对该方法进行验证。结果表明:该方法线性关系良好,相关系数为0.995 9~0.999 9,微量元素的检出限小于0.1μg/L。测定值均在标准值范围内,连续测定6次相对标准偏差(RSD)为0.1%~5.4%,回收率为82.7%~112.8%。11批葡萄籽提取物中,K、Ca、Mg、Na、Fe元素含量高,Al、Mn、Ni、Cu、B、Zn、Rb、Sr、Sn、Ba、Mo元素相对较高,Pb、As、Hg、Cd、Cr及15种稀土元素含量较低。验证结果表明,该方法专属性强、灵敏度高。

A microwave digestion-inductively coupled plasma mass spectrometry(ICP-MS) method was developed for the determination of 47 trace elements in grape seed extract by isotope, the method was validated by the reference material of celery component analysis. The results showed that linearity of the proposed method was good, the correlation coefficient(R) was 0.995 9–0.999 9, and the detection limit of trace elements was less than0.1 μg/L. The relative standard deviation(RSD) was 0.1% –5.4% for 6 successive times and the recovery was82.7%–112.8%. The contents of K, Ca, Mg, Na, Fe were the highest, the content of Al, Mn, Ni, Cu, B, Zn, Rb, Sr,Sn, Ba, Mo were relatively higher, and the contents of Pb, As, Hg, Cd, Cr and 15 rare earth elements were relatively low in 11 lots of grape seed extracts. Therefore, the method is specific and sensitiv.