氯离子通道基因MhCLC-e2促进缺氮条件下苹果愈伤组织的生长

Chloride channel gene MhCLC-e2 promotes the growth of apple calli under nitrogen deficiency

以苹果砧木平邑甜茶(Malus hupehensis var. pingyiensis) cDNA为模板,克隆了氯离子通道基因MhCLC-e2;该基因编码786个氨基酸,与桃PpCLCe的亲缘关系最近。将MhCLC-e2转入‘王林’苹果愈伤组织,获得3个MhCLC-e2过表达愈伤株系;在缺氮培养基中, 3株转基因愈伤组织生长状态均好于野生型,鲜重高于野生型,硝态氮、亚硝态氮、铵态氮和总氮含量均低于野生型,氨基酸含量高于野生型,显示MhCLC-e2可能通过影响氮素含量而促进缺氮条件下愈伤组织的生长。

The MhCLC-e2,chloride channel gene,was cloned from apple rootstock Malus hupehensis var.pingyiensis cDNA.MhCLC-e2 encoded 786 amino acids and had the closest genetic relationship with Pp CLCe in peach.We obtained three Mh CLC-e2 overexpressing apple calli.Under nitrogen-deficient medium,the growth potential of three MhCLC-e2 overexpressing apple calli were better than the wild-type,the contents of nitrate nitrogen,nitrite nitrogen,ammonium nitrogen and total nitrogen were lower than the wild-type,while the content of amino acids were higher than the wild-type.These results suggest that MhCLC-e2 may promote apple calli growth by influencing nitrogen content under nitrogen deficiency.