香根草对磺胺类抗生素污染水体的修复潜力研究

Studies on Phytoremediation Potentials of Vetiveria zizanioides to Sulfonamides Polluted Water

为探究香根草对水体中磺胺类抗生素的净化潜力,在温室水培模拟试验条件下,采用MCX固相萃取小柱的前处理方法,以高效液相色谱-串联质谱联用仪(LC-MS)为检测仪器,添加初始浓度为100μg·L^(-1)的磺胺类抗生素(SAs),分析香根草及营养液中SAs的残留动态。和无植物组相比,香根草栽植第15天对磺胺吡啶(SPY)、磺胺甲噁唑(SMZ)、磺胺二甲氧嘧啶(SDM)和磺胺喹啉(SQX)的去除率分别提高27.88%、32.81%、29.9%和20.03%。在15 d的培养试验中,香根草对SAs的吸收在第6天和12天出现2次高峰,栽植香根草营养液中的SAs在1~6 d去除率较高。处理时间与营养液中SAs的残留浓度呈极显著负相关(P<0.01),与水体中的去除率呈极显著正相关(P<0.01);转移系数与香根草茎叶、根中的SAs浓度呈极显著正相关(P<0.01)。根系是香根草积累SAs的主要器官组织。香根草对SDM有较高的吸收能力,对SMZ、SQX有较强的根部富集能力,对SPY有较好的转运能力。综上所述,种植香根草可显著提高水体中SAs的去除率,具有较好的净化潜能。

In order to investigate the phytoremediation potentials of Vetiveria zizanioide s to sulfonamides polluted water,the trials with the pretreatment method of MCX solid-phase extraction column and high performance liquid chromatography-tandem mass spectrometry(HPLC-MS)detection method were implemented under the condition of greenhouse hydroponics.Sulfonamides(SAs)were added to the nutrient solution with the initial concentration of 100μg·L^(-1).The results show that the removal rates of Vetiveria zizanioides to sulfonamides(SAs)increased by 27.88%for sulfapyridine(SPY),32.81%for sulfamethoxazole(SMZ),29.9%for sulfadimethoxine(SDM)and 20.03%for sulfaquinoline(SQX),in comparison of no Vetiveria zizanioides treatment for the 15th day.The absorption peaks of SAs by Vetiveria zizanioides were on the 6th and 12th day,and the decrease rates of SAs in Vetiveria zizanioides nutrient solution were higher in the the first 6 days.There was a significant negative correlation between the treatment time and the residual concentration of SA_(s) in nutrient solution(P<0.01),and a significant positive correlation between the treatment time and the increasing proportion of the removal rates in water(P<0.01).The transfer coefficients were significantly positive correlated with the concentration of SA_(s) in the stems,leaves and roots of Vetiveria zizanioides(P<0.01).Root systems of Vetiveria zizanioides were the main organ tissues in accumulating SAs.In conclusion,Vetiveria zizanioides are of efficient absorbing capability to SDM,strong root enrichment ability to SMZ and SQX,and efficient transferring ability to SPY.Vetiver zizanioides can significantly remove SAs in polluted water.