基于免疫学微流控芯片快速检测病原体的研究进展

Research progress of immunology-based microfluidic chip for rapid detection of pathogens

食品安全一直受到人们的极大关注,病原微生物的早期快速检测能够避免食源性疾病的大规模爆发。基于免疫学的微流控检测芯片是一种灵敏的、简便的、易于使用的检测平台,已广泛应用于病原微生物的快速检测中。与传统免疫学检测技术相比,免疫微流控检测芯片技术具有检测速度快、样本及试剂消耗少、高通量、功能集成化和自动化分析等特点。该文分别介绍了硅基、聚合物和纸基等免疫微流控检测芯片在病原微生物快速检测上研究进展,着重从检测性能及加工设计等方面分析了各芯片的优缺点。同时,讨论了免疫学微流控检测芯片目前面临的挑战和机遇,展望了该技术未来的发展趋势。

Food safety has always received great attention from people,early and rapid detection of pathogenic microorganisms can avoid large-scale outbreaks of food-borne diseases.The microfluidic detection chip based on immunology is a sensitive,simple and easy-to-use detection platform,which has been widely used in the rapid detection of pathogenic microorganisms.Compared with traditional immunological detection,the microfluidic detection chip has the characteristics of fast detection speed,less consumption of samples and reagents,high throughput,functional integration and automated analysis.This article focuses on the research progress of silicon-based,polymer and paper-based immune microfluidic detection chips from the aspects of chip design,manufacturing process,surface modification,etc.,and analyzes the advantages and disadvantages of each chip.At the same time,the current challenges and opportunities of the immunological microfluidic detection chip are discussed,and the future development trend of this technology is prospected.