山西平陆野生葛根悬浮细胞培养体系的优化

Optimization of Suspension Cell Culture System of Wild Pueraria Lobata in Pinglu County

对山西平陆野生葛根悬浮细胞培养过程所涉及的基本培养基、植物激素、pH值等3个影响因素进行了优化。结果表明,最适宜悬浮细胞生长的培养条件为:B5+1.0 mg/L 2,4-D+1.0 mg/L 6-BA+1.0 mg/L NAA+0.5 mg/L KT+30 g/L蔗糖;最适宜野葛根悬浮细胞合成总黄酮的最佳培养条件为:B5+2.0 mg/L 2,4-D+1.0 mg/L 6-BA+1.0 mg/L NAA+0.2 mg/L KT+40 g/L蔗糖,pH为5.8。

This paper optimizes the three influencing factors involved in the suspension cell culture of wild Pueraria lobata in Pinglu County,such as basic medium,plant hormone and pH.The results shows that the optimum culture conditions for the growth of suspension cells are B5+1.0 mg/L 2,4-D+1.0 mg/L 6-BA+1.0 mg/L NAA+0.5 mg/L KT+30 g/L sucrose.The optimum culture conditions for the synthesis of total flavonoids by Pueraria lobata suspension cells are B5+2.0 mg/L 2,4-D+1.0 mg/L 6-BA+1.0 mg/L NAA+0.2 mg/L KT+40 g/L sucrose,pH 5.8.