TCERG1L在乳腺癌中的表达及其与ERα的相互调控

Expression of TCERG1L in breast cancer and its regulation with ERα

目的探讨转录伸长调节因子1-like(Transcription elongation regulator 1-like,TCERG1L)在正常乳腺上皮细胞、导管原位癌细胞和浸润性导管癌(乳腺非特殊型浸润性癌)细胞中表达的异同,并探究其与ERα(Estrogen Receptorα)基因的相互调控。方法免疫组化评估61例乳腺导管原位癌(Ductal Carcinoma In Suit,DCIS)、60例乳腺浸润性导管癌(Invasive Ductal Carcinoma,IDC)和60例良性乳腺病变组织中TCERG1L及ERα的表达情况。Western blot检测TCERG1L和ERα在正常乳腺上皮细胞株HBL-10和乳腺癌细胞株MCF-7、MDA-MB-231中的表达并分析二者的相互调控关系。结果与正常乳腺上皮细胞对比,乳腺导管原位癌和浸润性导管癌细胞中TCERG1L的表达显著降低,组织切片及细胞株检测均显示TCERG1L与ERα相互抑制彼此的表达。结论TCERG1L表达显著降低是ERα阳性的乳腺浸润性导管癌的特征。TCERG1L有助于ERα阳性乳腺癌的鉴别诊断。

Objective We investigated the expression of Transcription elongation regulator 1-like(TCERG1L)in normal breast epithelial cells,ductal carcinoma in situ(DCIS)cells,and IDC cells,to investigate its correlation with ERα(Estrogen Receptorα).Methods Sixty-one cases of DCIS,60-cases of invasive ductal carcinoma(IDC),and 60 pathological benign breast masses were enrolled and assessed.The normal breast epithelial cell line HBL-10 and breast carcinoma cell lines MCF-7 and MDA-MB-231 were also investigated.TCERG1L and ERαexpression were evaluated by immunohistochemistry in tissue samples and western blot analysis in cultured cells.Results TECRG1L was highly expressed in normal breast ductal epithelial cells and ductal epithelial cells of pathologically benign breast masses.However,TECRG1L expression decreased significantly in DCIS/IDC cells,and was inversely correlated with ERαlevels.Conclusion In summary,reduced TCERG1L expression is characteristic of ERαpositive IDC.When distinguishing IDC from other lesion,TECRG1L expression represents a useful diagnostic.