中国无尾两栖类新记录种——朝鲜侧褶蛙

New Record Species of Anura from China——Pelophylax chosenicus

2021年9月在沈阳市蒲河湿地公园(41°41′54″N,122°52′19″E,海拔35 m)采集到3号无尾两栖类动物,经形态特征比较确认为侧褶蛙属(Pelophylax)金线侧褶蛙种组(P.plancyi species complex)的物种。基于线粒体12S rRNA和16S rRNA基因联合构建的侧褶蛙属部分物种最大似然系统发育树显示,采集到的侧褶蛙标本与朝鲜侧褶蛙(P.chosenicus)聚为一支,且具有较高的支持率(0.95)。综合形态和系统发育比较,确定采集到的标本为无尾目(Anura)蛙科(Ranidae)侧褶蛙属的朝鲜侧褶蛙,系中国两栖动物分布新记录种。

Three tailless amphibian specimens were collected in Puhe Wetland Park(41°41′54″N,122°52′19″E,elevation 35 m)in Shenyang in September 2021(Fig.1,with Fig.3 showing the habitat).In this study,the morphological characteristics of the collected specimens were compared and analyzed in detail with P.chosenicus and P.plancyi.The 12S rRNA and 16S rRNA gene of 3 samples were amplified and sequenced by second-generation high-throughput sequencing.The 12S rRNA and 16S rRNA gene information of 19 species of Pelophylax distributed in China and abroad(including a species of Rana as an outgroup)were obtained in NCBI GenBank database and existing reports.Phyml 3.0 to construct maximum likelihood phylogenetic tree.Morphological analysis shows that there are interspecific differences between our specimens and P.plancyi,which is consistent with the type origin species of P.chosenicus.The results of ML phylogenetic tree show that the Pelophylax specimens collected in Shenyang Puhe Wetland Park and the type origin(Korea)specimens of P.chosenicus gather into one branch(Fig.2),with a high support rate(95%).By comprehensive comparison with morphological and phylogenetic analysis,the specimens we collected should be the P.chosenicus of the genus Pelophylax in the family Anura,and be a new record of amphibian distribution in China.