百两金根的化学成分研究

Chemical Constituents from the Roots of Ardisia crispa(Thunb.)A.DC.

目的百两金为传统苗药,其化学成分的有关研究报道较少,本研究目的在于研究百两金的化学成分。方法将百两金干燥根体积分数70%乙醇提取物利用硅胶、重结晶、ODS及制备/半制备HPLC进行分离纯化,根据理化性质及波谱数据对所得化合物结构进行鉴定。结果从百两金根中得到了20个化合物。分别为岩白菜素(1)、甲基岩白菜素(2)、11-O-没食子酰岩白菜素(3)、syringin(4)、(+)-syringaresinol-O-β-D-glucopyranoside(5)、(-)-(7R,8S,7'R,8'S)-4,9,4',9'-tetrahydroxy-3,3'-dimethoxy-7,7'-epoxylignan-9-O-β-D-xylopyranoside(6)、saracoside(7)、isolariciresinol-4-O-β-D-glucopyranoside(8)、(7S,8R)-urolignoside(9)、staphylionoside D(10)、蚱蜢酮(11)、l-borneol 6-O-β-D-aiosyl-β-Dglucoside(12)、(+)-angelicoidenol 2-O-β-D-glucopyranoside(13)、牡荆素-2″-O-鼠李糖苷(14)、根皮苷(15)、phenethyl alcoholβ-D-(2'-O-β-D-glucopyranosyl)glucopyranoside(16)、phenylethyl-β-D-glucopyranoside(17)、菖蒲碱C(18)、己内酰胺(19)、蔗糖(20)。结论其中化合物5~19为首次从紫金牛科植物中分离得到,化合物4为首次从紫金牛属植物中分离得到,化合物2和3为首次从该植物中分离得到。其中化合物6是自国内学者2013年从岭南杜鹃分离得到以来第二次被分离得到。

OBJECTIVE To enrich the chemical constituents of Ardisia crispa(Thunb.)A.DC..METHODS The 70%ethanol extract of Ardisia crispa(Thunb.)A.DC.was isolated and purified by silica,recrystallization,ODS and preparative/semi-preparative HPLC,and the structures of obtained compounds were identified by physico-chemical properties and spectroscopic data.RESULTS The compounds are bergenin(1),methylbergenin(2),11-O-galloylbergenin(3),syringin(4),(+)-syringaresinol-O-β-D-glucopyranoside(5),(-)-(7R,8S,7'R,8'S)-4,9,4',9'-tetrahydroxy-3,3'-dimethoxy-7,7'-epoxylignan-9-O-β-D-xylopyranoside(6),saracoside(7),isolariciresinol-4-O-β-D-glucopyranoside(8),(7S,8R)-urolignoside(9),staphylionoside D(10),grasshopper ketone(11),l-borneol 6-O-β-D-aiosyl-β-D-glucoside(12),(+)-angelicoidenol-2-O-β-D-glucopyranoside(13),vitexin-2″-O-rhamnoside(14),phlorizin(15),phenethyl alcohol-β-D-(2'-O-β-D-glucopyranosyl)glucopyranoside(16),phenylethyl-β-D-glucopyranoside(17),tatarine C(18),caprolactam(19)and sucrose(20).CONCULUSION Compounds 5-19 are firstly isolated from the family Myrsinaceae,compound 4 is isolated from genus Ardisia for the first time and compound 2 and 3 are obtained from this plant for the first time.Compound 6 is the second occurrence ever domestic scholars obtained it from Rhododendron mariae Hance in 2013.