RP-HPLC法分离[口恶]唑酰草胺对映异构体

Chiral separation of metamifop enantiomers by RP-HPLC

[目的]建立一种利用高效液相色谱测定手性农药[口恶]唑酰草胺中光学异构体的分析方法。[方法]通过对[口恶]唑酰草胺流动相的种类、流速、柱温和其他流动相的比例等因素进行了分离效果的综合考察,最终优化了[口恶]唑酰草胺的分离条件。[结果]通过试验,其优化的分离条件为:使用Caprichiral ADS-H手性柱,以甲醇与水(体积比95∶5)作为流动相,流速、进样量和柱温分别是0.5 mL/min、5μL和30℃,达到了2.65的分离度。[结论]该方法分离效果好,可用于[口恶]唑酰草胺光学异构体的光学纯度分析。

[Aims] This study aims to establish an analytical method for the determination of optical isomers in chiral pesticide metamifop by RT-HPLC. [Methods] Through the comprehensive investigation of the separation effect of metamifop mobile phase, flow rate, column temperature and the proportion of other mobile phases, the separation conditions of metamifop were optimized. [Results] The best separation conditions were as follows: using Caprichiral ADS-H chiral column, methanol and water(95:5, by vol) as mobile phase, the flow rate, injection volume and column temperature were 0.5 mL/min and 5 μL and 30 ℃ respectively, reaching a resolution of 2.65. [Conclusions] The method has good separation effect, high precision, high efficiency and accuracy. It can be used for the optical purity analysis of metamifop optical isomers.