IL-6表达水平及其基因启动子区多态性与脓毒症的相关性研究

Correlation of IL-6 expression level and its gene promoter polymorphism with sepsis

目的探讨脓毒症患者外周血白细胞介素-6(IL-6)表达水平及脓毒症进展与IL-6基因启动子区-174G/C、-572C/G和-597G/A位点多态性间的关系。方法选择2018年1月至2021年1月重钢总医院收治的脓毒症患者113例为观察组,选取同期该院体检健康人群132例作为对照组,并根据第3版脓毒症诊断标准和分型方法,将113例脓毒症患者分为脓毒症组(n=51)和脓毒症休克组(n=62);采集各组受试者外周血,检测血浆中IL-6和降钙素原(PCT)表达水平,采用聚合酶链式反应-限制性内切酶片段长度多态性(PCR-RFLP)技术检测IL-6基因启动子区-174G/C、-572C/G、-597G/A位点多态性位点单核苷酸多态性(SNPs),分析以上基因多态性与脓毒症易感性及其进展的关系。结果观察组IL-6和PCT的血浆水平明显高于对照组(P<0.001),且脓毒症休克组IL-6和PCT的血浆水平明显高于脓毒症组(P<0.001)。与对照组比较,观察组IL-6基因多态性位点-572C/G的基因型CC及等位基因C频率明显偏低(P<0.05);与脓毒症组比较,脓毒症休克组IL-6基因多态性位点-572C/G的基因型CC及等位基因C频率明显偏低(P<0.05),而-174G/C和-597G/A位点比较差异无统计学意义(P>0.05);IL-6基因-572C/G位点基因型为CC的脓毒症患者血浆IL-6和PCT水平明显低于基因型为CG/GG患者(P<0.001)。结论IL-6基因-572C/G多态性与脓毒症的发病风险具有相关性,CC基因型为脓毒症的保护基因,GG基因型为易感基因型,且其多态性影响IL-6表达水平。

Objective To explore the correlation of peripheral blood interleukin-6(IL-6)expression level and septic progress with IL-6 gene promoter regions-174G/C,-572C/G and-597G/A loci polymorphisms.Methods A Total of 113 patients with sepsis treated in Chonggang General Hospital from January 2018 to January 2021 were collected as the observation group,and contemporaneous 132 healthy subjects who underwent physical examination in this hospital were selected as the control group.The patients with sepsis were divided into the sepsis group(n=51)and septic shock group(n=62)according to the third edition of sepsis diagnostic standard and classification method.The peripheral blood of subjects in each group was collected.The expression levels of plasma IL-6 and procalcitonin(PCT)were detected.The single nucleotide polymorphisms(SNPs)of IL-6 gene promotor regions-174G/C,-572C/G and-597G/A loci were detected by the PCR-restriction fragment length polymorphism(PCR-RFLP).The relationship between the above gene polymorphisms and sepsis susceptibility and progression was analyzed.Results The plasma IL-6 and PCT levels in the observation were significantly higher than those in the control group(P<0.001),moreover the plasma IL-6 and PCT levels in the septic shock group were significantly higher than those in the sepsis group(P<0.001).Compared with the control group,the IL-6 genotypes CC at-572C/G gene polymorphism loci and the allele C frequency in the observation group were significantly lower(P<0.05),while compared with the sepsis group,the IL-6 genotypes CC at-572C/G and the allele C frequency in the septic shock group were significantly lower(P<0.05).No statistically significant difference was found in-174G/C and-597G/A loci between these groups(P>0.05).The plasma IL-6 and PCT levels in sepsis patients with CC genotype at IL-6 gene-572C/G loci were significantly lower than those in patients with CG/GG genotype(P<0.001).Conclusion The IL-6 gene polymorphism at-572C/G loci has the correlation with the onset risk of sepsis,the CC genotype is a protective gene of sepsis,the GG genotype is a susceptible genotype,moreover their polymorphism affects the IL-6 expression level.