miR-182-5p通过靶向调节KLF7抑制骨肉瘤细胞的增殖、迁移和侵袭

miR-182-5p inhibits proliferation,migration and invasion of osteosarcoma cells by targeting KLF7

目的:研究miR-182-5p对人骨肉瘤细胞增殖、迁移和侵袭的作用。方法:用qRT-PCR检测法比较人骨肉瘤细胞系(U2OS、MG63、SAOS2)、人正常细胞和人成骨细胞(HFOB1.19)中miR-182-5p的表达水平。转染miRNA模拟物或抑制剂或模拟物+KLF7,以转染miR-182-5p表达的上调或下调。通过EDU、迁移分析和侵袭分析来检测细胞功能。双荧光素酶报告分析检测miR-182-5p与KLF7的关系,蛋白质印迹分析检测KLF7的表达。结果:miR-182-5p在骨肉瘤细胞系中被下调。miR-182-5p过表达抑制肿瘤生长、迁移和侵袭。随后的研究显示,KLF7是骨肉瘤细胞中miR-182-5p的直接和功能靶点。miR-182-5p通过调节KLF7来抑制骨肉瘤细胞的增殖、迁移和侵袭。结论:miR-182-5p通过靶向调节KLF7而起到抑制骨肉瘤细胞增殖、迁移和侵袭的作用。

Objective:To explore the effect of miR-182-5p on osteosarcoma cells proliferation,migration and invasion.Methods:The miR-182-5p expression level in osteosarcoma cell lines(U2OS,MG63,SAOS2),normal cells and human normal osteoblastic cell line(hFOB1.19)was detected by qRT-PCR.miRNA mimics or inhibitor,miRNA mimics plus KLF7 were transfected for up-regulation or down-regulation of miR-182-5p expression.Cell function was assayed by EDU,migration assay and invasion assay.The relation between miR-182-5p and KLF7 was measured by luciferase reporter gene assay.The expression level of KLF7 was detected using Western blot.Results:miR-182-5p was down-regulated in osteosarcoma cell lines.Overexpression of miR-182 inhibited tumor growth,migration and invasion.Subsequent investigation revealed that KLF7 was a direct and functional target of miR-182-5p in osteosarcoma cells.miR-182-5p inhibited the proliferation,migration and invasion by regulating KLF7 in osteosarcoma cells.Conclusion:miR-182-5p acts as a suppressor by targeting KLF7 in osteosarcoma.