京尼平苷通过调节Perilipin-2表达对肝细胞脂滴形成的影响

Effects of geniposide on lipid droplet formation in hepatocytes via regulation of Perilipin-2 expression

目的 探究京尼平苷对肝细胞脂滴积累和脂滴相关蛋白Perilipin-2表达的影响。方法 采用葡萄糖耐量实验检测ob/ob小鼠的空腹血糖和糖耐量,Vitros-250化学分析仪检测血清甘油三酯(TG)和总胆固醇(TC)水平,HE、油红O染色检测ob/ob小鼠肝组织及油酸诱导的HepG2细胞中脂滴形成情况,RT-qPCR和Western blot法检测ob/ob小鼠肝组织和油酸处理的HepG2细胞中Perilipin-2 mRNA和蛋白表达。结果 京尼平苷可改善ob/ob小鼠胰岛素抵抗(P<0.05),降低ob/ob小鼠体质量、血清中TG和TC水平(P<0.05,P<0.01),减少肝组织中脂滴数量和大小,抑制脂滴相关蛋白Perilipin-2表达(P<0.05)及油酸诱导的HepG2细胞中脂滴的积累和Perilipin-2表达(P<0.05,P<0.01)。结论 京尼平苷可能通过抑制Perilipin-2表达减少脂滴的积累,进而改善非酒精性脂肪性肝病症状。

AIM To explore the effects of geniposide on the accumulation and expression of related protein Perilipin-2 in lipid droplet of hepatocytes.METHODS The mice were subjected to the detection of fasting blood glucose and glucose tolerance of ob/ob by glucose tolerance test;the detection of serum triglyceride(TG) and total cholesterol(TC) levels by Vitros-250 chemical analyzer;and the determination of ob/ob mice and oleic acid-treated HepG2 cells about the formation of lipid droplets by HE and oil red O staining,and about mRNA and Perilipin-2 protein expressions by RT-qPCR and Western blot.RESULTS To ob/ob mice,geniposide improved their insulin resistance(P<0.05);reduced their body weights and serum TG and TC levels(P<0.05,P<0.01).Once treated by geniposide,lipid droplets in the liver tissues were reduced in number and size,inhibited in Perilipin-2 protein expressions(P<0.05);and in contrast to the inhibition of accumulation and Perilipin-2 expressions of lipid droplets in oleic acid-induced HepG2 cells(P<0.05,P<0.01).CONCLUSION Geniposide may improve the symptoms of non-alcoholic fatty liver disease due to its efficacy in the reduction of lipid droplets accumulation via inhibition of Perilipin-2 expression.