洋金花叶化学成分及其抗炎活性研究

Chemical constituents from the leaves of Datura metel and their anti-inflammatory activities

目的 研究洋金花Datura metel L.叶化学成分及其抗炎活性。方法 采用大孔吸附树脂、硅胶、ODS、HPLC法对洋金花叶70%乙醇提取物进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构,采用RAW264.7模型评价其体外抗炎活性。结果 从中分离得到28个化合物,分别鉴定为7,27-dihydroxy-1-oxowitha-2,5,24-trienolide(1)、acnistoferin(2)、baimantuoluoline K(3)、daturafoliside G(4)、daturafoliside H(5)、daturafoliside I(6)、daturafoliside R(7)、daturafoliside S(8)、daturametelin A(9)、daturametelin I(10)、daturametelin J(11)、daturataturin A(12)、baimantuoluoside H(13)、(22R)-27-hydroxy-7α-methoxy-1-oxowitha-3,5,24-trienolide(14)、柯里拉京(15)、desmethylagrimonolide 6-O-β-D-glucopyranoside(16)、仙鹤草内酯-6-O-β-D-吡喃葡萄糖苷(17)、丁香脂素(18)、丁香脂素-4-O-β-D-吡喃葡萄糖苷(19)、1,6-di-O-coumaroyl glucopyranoside(20)、滨蒿内酯(21)、东莨菪内酯(22)、野蔷薇亭(23)、(-)-loliolide(24)、(+)-isololiolide(25)、邻苯二甲酸二丁酯(26)、对羟基苯甲醛(27)、没食子酸乙酯(28)。化合物9、20能抑制LPS诱导细胞释放NO,IC_(50)分别为25.14、16.26μmol/L。结论 化合物15~17、20、23、28为首次从茄科植物中分离得到,化合物2、19、24、27首次从曼陀罗属植物中分离得到。化合物9、20具有较强的抗炎活性。

AIM To study the chemical constituents from the leaves of Datura metel L.and their anti-inflammatory activities.METHODS The 70% ethanol extract of the leaves of D.metel was isolated and purified by macroporous resin,silica gel,ODS and HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.Their anti-inflammatory activities in vitro were evaluated by RAW264.7 model.RESULT Twenty eight compounds were isolated and identified as 7,27-dihydroxy-1-oxowitha-2,5,24-trienolide(1),acnistoferin(2),baimantuoluoline K(3),daturafoliside G(4),daturafoliside H(5),daturafoliside I(6),daturafoliside R(7),daturafoliside S(8),daturametelin A(9),daturametelin I(10),daturametelin J(11),daturataturin A(12),baim antuoluoside H(13),(22R)-27-hydroxy-7α-methoxy-1-oxowitha-3,5,24-trienolide(14),corilagin(15),desmethylagrimonolide 6-O-β-D-glucopyranoside(16),agrimonolide-6-O-β-D-glucopyranoside(17),syringaresinol(18),syringaresinol-4-O-β-D-glucopyranoside(19),1,6-di-O-coumaroyl-glucopyranoside(20),scoparone(21),scopoletin(22),rosamultin(23),(-)-loliolide(24),(+)-isololiolide(25),dibutyl phthalate(26),p-hydroxybenzaldehyde(27),ethyl gallate(28).Compounds 9,20 effectively inhibited the production of NO by LPS-induced cells with the IC_(50) value of 25.14,16.26 μmol/L,respectively.CONCLUSION Compounds 15-17,20,23,28 are first isolated from family Solanaceae,compounds 2,19,24,27 are isolated from genus Datura for the first time.Compounds 9 and 20 show strong anti-inflammatory activities.