甲基营养型芽孢杆菌NKG-1可湿性粉剂的制备及药效研究

Bacillus methylotrophicus NKG-1 Wettable Powder: Preparation and Efficacy Study

为了制备甲基营养型芽孢杆菌NKG-1可湿性粉剂,评价其防病促生效果。试验以NKG-1为试材,通过筛选载体和助剂,利用混料设计确定可湿性粉剂的最优配方,并进行药效测定。结果表明:NKG-1可湿性粉剂的最佳载体为白炭黑,最佳助剂为烷基萘磺酸盐甲醛缩聚物和嵌段共聚物混合物(Morwet D-500)及烷基萘磺酸盐(Petro AA)。混料设计确定了最优质量配比为:白炭黑:Morwet D-500:Petro AA=72:16:12。药效试验结果表明:NKG-1可湿性粉剂100倍液处理番茄植株后,其鲜重、干重、株高以及根长分别较对照增加了53.34%、61.01%、88.02%、78.53%,对番茄灰霉病的平均防治效果高达82.02%。本研究确定了甲基营养型芽孢杆菌NKG-1可湿性粉剂的最优配比,评测了制剂的药效,为NKG-1的生产应用奠定了试验基础。

In order to prepare wettable powder of Bacillus methylotrophicus NKG-1 and evaluate its effect on disease prevention and growth promotion, we used NKG-1 as the test material, determined the optimal formula of the wettable powder(WP) through the selection of carrier and auxiliary agent by the mixture design, and conducted the efficacy evaluation on the WP. The results showed that the best carrier of NKG-1 WP was silica,and the best additives were the mixture of alkyl naphthyl sulfonate formaldehyde condensation and block copolymer(Morwet D-500) and alkyl naphthalene sulfonate(Petro AA). The mixture design determined the optimal mass ratio: silica: Morwet D-500: Petro AA=72:16:12. The control efficacy test results showed that the fresh weight, dry weight, plant height and root length of tomato plants treated with NKG-1 wettable powder 100times solution increased by 53.34%, 61.01%, 88.02% and 78.53%, respectively, compared with those of the control. The average control effect of NKG-1 wettable powder on Botrytis cinerea could reach 82.02%. In this study, the optimal ratio of Bacillus methylotrophicus NKG-1 WP was determined, and the efficacy of the preparation was evaluated, which could lay an experimental foundation for the production and application of NKG-1.