IL-6促进大鼠星形胶质细胞活化并下调内向整流钾离子通道4.1(Kir4.1)的表达

IL-6 promotes the activation of rat astrocytes and down-regulation of the expression of Kir4.1 channel

目的探讨白细胞介素6(IL-6)对大鼠星形胶质细胞(Ast)的活化作用及其对内向整流钾离子通道4.1(Kir4.1)表达的影响。方法分离培养新生SD大鼠的大脑皮层Ast,用IL-6或联合IL-6受体拮抗剂(IL-6Ra)处理培养的Ast,CCK-8法检测细胞活力;实时荧光定量PCR和Western blot法分别检测IL-6对Ast Kir4.1通道的mRNA和蛋白表达的影响。结果IL-6在一定剂量(≤30 ng/mL)和作用时间(≤24 h)范围内可促进Ast的活化;用30 ng/mL的IL-6处理Ast 24 h后,Kir4.1通道的mRNA和蛋白表达均显著下降,但此作用可被IL-6Ra所逆转。结论IL-6可促进Ast活化和下调Kir4.1通道的表达。

Objective To identify the effects of interleukin-6(IL-6)on astrocytes activation,and the regulation of the expression of inwardly rectifying potassium 4.1(Kir4.1)channels in astrocytes.Methods Astrocytes were separated from the cerebral cortex of newborn SD rats,and cultured in the presence of IL-6 or combined with interleukin-6 receptor antagonist(IL-6Ra).CCK-8 assay was performed to measure cell viability.The expression level of Kir4.1 channels in astrocytes was measured using quantitative real-time PCR and Western blot analysis.Results IL-6 promoted the proliferation of astrocytes in a dose-(0-30 ng/mL)and time-(0-24 hours)dependent manner.After astrocytes were treated with IL-6(30 ng/mL)for 24 hours,the levels of Kir4.1 mRNA and protein decreased significantly,and this down-regulation could be attenuated by IL-6Ra.Conclusion IL-6 promotes the activation of astrocyte and down-regulation of the expression of Kir4.1 channel.