幽门螺杆菌阳性萎缩性胃炎大鼠生物信息分析及安胃汤影响的研究

Bioinformatics Analysis of Helicobacter pylori-Positive Rats with Atrophic Gastritis and the Effect of Anwei Decoction

目的:基于生物医学大数据筛选幽门螺杆菌(Hp)相关性慢性萎缩性胃炎的关键基因,探索其发病机制及安胃汤对其的影响。方法:从数据库Gene Expression Omnibus(GEO)下载Hp相关性慢性萎缩性胃炎相关芯片数据,利用GEO_(2)R软件筛选出Hp相关性慢性萎缩性胃炎差异基因,进行生物信息学分析,根据蛋白参与通路数量确定核心蛋白,建立Hp阳性慢性萎缩性胃炎大鼠模型,用PCR和蛋白质印迹法(Western Blotting)进行检测并观察安胃汤对其表达的影响。结果:经过检索分析GSE13873和GSE27411系列芯片数据被纳入,取2个芯片交集的20个差异基因进行生物信息学分析,发现载脂蛋白A1、CD36、囊性纤维化穿膜传导调节蛋白(CFTR)可能是信号通路的核心蛋白。Western Blotting及PCR结果显示Hp相关性慢性萎缩性胃炎大鼠胃组织中,CD36蛋白表达下调(P<0.05),载脂蛋白A1和CFTR蛋白表达上调(P<0.05);与模型组比较,实验观察高剂量组、实验观察中剂量组和实验观察低剂量组胃组织中CD36蛋白表达上调,载脂蛋白A1和CFTR表达下调(P<0.05),其与Hp相关性慢性萎缩性胃炎发病关系密切。结论:通过对GEO中Hp相关性慢性萎缩性胃炎芯片生物信息学分析结合Western Blotting及PCR进一步的验证发现载脂蛋白A1、CD36、CFTR作为信号通路的核心蛋白实验结果与生物信息学分析结果一致。

Objective:To screen key genes of Helicobacter pylori(Hp)-related chronic atrophic gastritis based on biomedical big data and explore its pathogenesis and the influence of Anwei Decoction.Methods:The chip data of Hp-related chronic atrophic gastritis were downloaded from Gene Expression Omnibus(GEO),and the differential genes of HP-related chronic atrophic gastritis were screened out by GEO2R.Bioinformatics analysis was conducted,and the core proteins were determined according to the number of protein-participating pathways.The Hp(+)chronic atrophic gastritis model was induced in rats.PCR and Western blot were used to detect the effect of Anwei Decoction on the expression.Results:After retrieval and analysis of GSE13873 and GSE27411 series chip data,20 common differential genes of the two chips were taken for bioinformatics analysis,and the results showed that apolipoprotein A1(APOA1),CD36,and cystic fibrosis transmembrane conductance regulator(CFTR)might be the core proteins of the signaling pathway.Western blot and PCR results showed that the expression of CD36 protein was down-regulated(P<0.05),and the expression of APOA1 and CFTR was up-regulated(P<0.05)in the gastric tissues of rats with HP-related chronic atrophic gastritis.Compared with the model group,the high-,medium-and low-dose Anwei Decoction groups showed up-regulated expression of CD36 and down-regulated expression of APOA1 and CFTR(P<0.05),which was closely related to the incidence of HP-related chronic atrophic gastritis.Conclusion:As revealed by the bioinformatics analysis of HP-related chronic atrophic gastritis in GEO microarray and verification by Western blot and PCR,the experimental results of APOA1,CD36,and CFTR as the core proteins of the signaling pathway were consistent with the results of bioinformatics analysis.