水稻TCP家族的全基因组鉴定及表达分析

Genome-wide Identification and Expression Analysis of TCP Family in Rice(Oryza sativa L.)

TCPs是植物特有的转录调控因子,参与发育和逆境响应等。本研究对水稻Os TCP基因家族进行了全面的生物信息学分析,并对这些基因在水稻非生物胁迫条件下的GEO表达谱数据进行了筛选和表达验证。结果表明:水稻Os TCP家族有27个成员,蛋白分子量在27~117 kD之间,等电点基本都小于5.2。根据蛋白质序列对比和结构域分析表明:27个基因可以分为TCP-P和TCP-C两类,含有TCP结构域。OsTCP基因结构均较为简单,内含子数目较少,大部分基因含有相同的motif 1和motif 2。顺式元件分析发现,水稻TCP家族基因的启动子序列中含多个非生物胁迫响应元件。此外,对GEO数据库的水稻表达谱数据筛选与基于荧光定量PCR技术的表达水平验证结果表明,候选的10个水稻OsTCP基因对高温、低温、盐和镉胁迫有显著的响应,说明其是响应逆境胁迫分子网络机制中的重要调控因子。本研究不仅加深了我们对水稻TCP家族的了解,更为水稻耐逆研究提供了重要的信息。

TCPs are plant-specific transcriptional regulators,which are involved in development and stress response.The study conducted a comprehensive bioinformatics analysis of the Os TCP gene family in rice,these genes were screened based on GEO expression profile database and their expression under abiotic stress conditions was verified.The results showed that there were 27 members from Os TCP family in rice,the molecular weight of these Os TCP proteins ranged from 27 kD to 117 kD,and the isoelectric points were basically less than 5.2.Sequence comparison and domain analysis showed that the 27 genes were divided into two types,TCP-P and TCP-C,containing TCP domain.The structure of OsTCP genes,with a small number of introns,were relatively simple,and most of them contained motif 1 and motif 2.The analysis of cis-elements found that the promoter sequences of OsTCP genes contained multiple abiotic stress response elements.In addition,the screening of GEO expression profile data and expression level validation by using the quantitative Real-time PCR(RT-qPCR)showed that the candidate 10 OsTCP genes in rice had significant responses to high temperature,low temperature,salt and cadmium stresses,indicating that they were important regulatory factors in response to stress molecular network mechanism.This study not only deepened our understanding of TCP family in rice,but also provided important information for stress resistance research in rice.