CASR/VDR/PTH1R信号通路在含钙肾结石发生机制中的初步研究

A Preliminary Study of CASR/VDR/PTH1R Signaling Pathway in the Pathogenesis of Calcium-containing Kidney Stones

目的研究CASR/VDR/PTH1R通路在含钙肾结石发生中的作用及机制。方法取肾结石患者肾脏髓质为实验组,肾脏肿瘤患者正常肾髓质为对照组,分别采用q-PCR、WB、免疫荧光法检测肾实体组织中CASR、VDR、PTH1R的mRNA表达和蛋白水平。结果CASR、PTH1R基因mRNA在积水肾髓质及肾肿瘤正常髓质表达水平差异无统计学意义(P>0.05)。VDR基因的mRNA在积水肾髓质及肾肿瘤正常髓质表达水平差异有统计学意义(P<0.05)。结论CASR、VDR、PTH1R在肾结石患者肾脏髓质中表达,且有可能通过CASR/VDR/PTH1R信号通路途径参与含钙肾结石的发生机制。

Objective To study the role and mechanism of CASR,VDR,PTH1R and other genes in the occurrence of calcium-containing kidney stones.Methods The renal medulla of patients with renal calculi was selected as the experimental group,and the normal renal medulla of patients with renal tumors was selected as the control group.The mRNA expression and protein level of CASR,VDR and PTH1R in renal solid tissue were detected by q-PCR,WB and immunofluorescence,respectively.Results There was no significant difference in the expression levels of CASR and PTH1R gene mRNA in the hydronephrotic medulla and the normal medulla of renal tumor(P>0.05).The difference of VDR gene mRNA expression in hydronephrotic medulla and normal medulla of renal tumor was statistically significant(P<0.05).Conclusion CASR,VDR,and PTH1R are involved in the occurrence of calcium-containing kidney stones,and may participate in the mechanism of calciumcontaining kidney stones through the CASR/VDR/PTH1R signaling pathway.