毛竹谷胱甘肽过氧化物酶基因分子特征及其表达模式分析

Molecular characteristics and expression pattern analysis of glutathione peroxidase genes in Phyllostachys edulis(Carriere)J.Houzeau

利用生物信息学以及分子生物学方法对毛竹(Phyllostachys edulis(Carriere)J.Houzeau)谷胱甘肽过氧化物酶基因(GPX)的分子特征以及表达模式进行分析。结果显示,在毛竹中共鉴定出9个GPX家族成员基因(PeGPX1-PeGPX9),均具有6个外显子以及5个内含子,PeGPXs编码蛋白的长度为168~235 aa,相对分子量在18.41~25.54 kD,等电点范围为5.88~9.48。亚细胞定位预测结果发现,除PeGPX5定位在线粒体上,其他PeGPXs都定位在叶绿体上。在PeGPXs启动子中含有多种与胁迫和激素相关的顺式作用元件。qRT-PCR分析结果表明,强光、低温、GA_(3)、NAA和MeJA处理均可引起毛竹叶片中PeGPXs表达量发生明显变化。

The molecular characteristics and expression patterns of glutathione peroxidase genes(GPX)in moso bamboo(Phyllostachys edulis(Carriere)J.Houzeau)were analyzed using bioinformatics and molecular biology.A total of nine GPX genes were identified from the genome of moso bamboo(PeGPX1-PeGPX9),which all contained six exons and five introns.The lengths of proteins encoded by PeGPXs ranged from 168 to 235 aa,with relative molecular weights of 18.41 to 25.54 kD and isoelectric points of 5.88 to 9.48.Subcellular localization analysis predicted that all PeGPXs were localized on the chloroplast,except for PeGPX5 on mitochondria.A variety of stress and hormone-related cis-acting elements were found in the promoters of PeGPXs.Quantitative real-time polymerase chain reaction(qRT-PCR)showed that the expression levels of PeGPXs in the leaves of moso bamboo were distinctly affected by high light,low temperature,GA_(3),NAA,and MeJA treatments.