水相法合成磷脂酰丝氨酸体系的优化研究

Optimization of the System for the Synthesis of Phosphatidylserine by Aqueous Method

本文对水相法合成磷脂酰丝氨酸在工业生产中的关键变量进行了研究。结果表明,水相法合成磷脂酰丝氨酸最佳体系为水量∶大豆磷脂∶丝氨酸的比例为3.0∶1.0∶0.8(mL∶g∶g)、酶液添加量为磷脂酰胆碱的20%、温度45℃、时间6 h。最终磷脂酰丝氨酸含量为54.52%,转化率高达93.4%。本研究为磷脂酰丝氨酸在工业化生产中的应用提供了重要参考依据。

In this paper, the key variables of phosphatidylserine synthesis by water phase method in industrial production were studied. The results showed that the optimum conditions for the synthesis of phosphatidylserine by water phase method were as follows: the ratio of water to soy phosphatidylserine was 3 ∶ 1 ∶ 0.8(mL ∶ g ∶ g),the dosage of enzyme was 20% of phosphatidylcholine, the temperature was 45 ℃, and the time was 6 h. The final phosphatidylserine content was 54.52% and the conversion rate was 93.4%. This study provides an important reference for the application of phosphatidylserine in industrial production.