罗汉果茎段组培快繁技术研究

Study on Tissue Culture and Rapid Propagation of Siraitia grosvenorii Stem

以青皮果品种的罗汉果幼嫩茎段作为试验材料,探讨不同消毒试剂、消毒时间、激素组合及激素浓度梯度对罗汉果组织培养过程中的影响,以期在短时间内获得大量罗汉果组培苗。结果表明:外植体最佳消毒方式为0.1%氯化汞溶液消毒6 min,成活率达85%;激素组合为MS+0.5 mg/L 6-BA+0.1 mg/L NAA的培养基可诱导发芽;激素组合为MS+0.4 mg/L IBA+1mg/L 6-BA为最佳继代增殖培养基,增殖系数达6.3;最佳芽苗伸长培养基为MS+0.05 mg/L 6-BA+0.1 mg/L GA_(3),35 d平均伸长5.1 cm;最佳生根培养基为1/2MS+0.5 mg/L IBA+0.5 mg/L NAA,生根数量在3~8条之间,生根率达86%。

The effects of different kinds of reagents and disinfection time,hormone combination and hormone concentration gradient on the tissue culture of Siraitia grosvenorii were studied by using tender stems as experimental materials.The best disinfection way for Siraitia grosvenorii is 0.1% HgCl_(2) solution disinfecting 6 min,efficiency of sterilization can reach 85%.The tender stems were cultured on the medium of 0.5 mg/L 6-BA+0.1 mg/L NAA,and the seedlings with large shoots were obtained,and the stem segments of the seedlings were removed as explants for further cultivation.It was found that the 0.4 mg/L IBA+1 mg/L 6-BA was the best subculture medium,and the multiplication coefficient was up to 6.3.The best culture medium of shoots development was 0.05 mg/L 6-BA+0.1mg/L GA_(3),the average elongation of 35 d was 5.1 cm.The best rooting medium was 1/2MS+0.5 mg/L IBA+0.5mg/L NAA,and the number of rooting was 3-8.Rooting rate was up to 86%.