云南主栽蓝莓果实花色苷组成差异分析

Analysis of Anthocyanin Composition Difference in Yunnan Main Cultivated Blueberry Fruits

本研究以云南澄江蓝莓主产区6个品种蓝莓鲜果为对象,利用高效液相色谱法(HPLC)和加标定性测定法检测花色苷组成,通过主成分分析法对花色苷组成进行差异分析。结果表明,6个品种的蓝莓果实中共检测到10种花色苷,其中,“比洛克西”的总花色苷含量最高,为2933.551 mg/kg,芍药素-3-O-葡萄糖苷和飞燕草素-3-O-半乳糖苷含量在“比洛克西”中均最高,分别为476.703 mg/kg和595.614 mg/kg。主成分分析表明不同品种蓝莓单体花色苷所占比例有明显差异,可以区分出花色苷含量丰富的“比洛克西”和“莱格西”两大类蓝莓品种和6个蓝莓品种中极为丰富的花色苷。综上可知,花色苷可以作为化学指纹区分6个不同品种的蓝莓,为筛选和培育优质蓝莓品种提供理论支撑。

In this study,the anthocyanins of 6 blueberry fresh fruits from Chengjiang,Yunnan Province were detected by HPLC and standard qualitative determination.The anthocyanins were analyzed by principal component analysis(PCA).The results showed that a total of 10 anthocyanins were detected in blueberry fruits of 6 varieties,among which the content of total anthocyanins in biloxi was the highest(2,933.551 mg/kg).And the content of paeoniflorin-3-O-glucoside(476.703 mg/kg)and Delphinin-3-O-galactoside were the highest in Biloxi(595.614mg/kg).Principal component analysis showed that the proportion of anthocyanins in different varieties of blueberries was significantly different.The anthocyanins of Biloxi and Legosi blueberry varieties with abundant anthocyanins could be distinguished from those of 6 blueberry varieties.In conclusion,anthocyanins can be used as chemical fingerprints to distinguish 6 different varieties of blueberry,thus providing theoretical support for screening and breeding high-quality blueberry varieties.