系统性红斑狼疮和原发性干燥综合征的共同基因表达特征和分子机制

The common genetic characteristics and molecular mechanisms of systemic lupus erythematosus and primary Sjogren’s syndrome

目的初步探究系统性红斑狼疮(systemic lupus erythematosus,SLE)和原发性干燥综合征(primary Sj9gren’s syndrome,pSS)的共同基因表达特征和分子机制。方法利用基因表达综合数据库(Gene Expression Omnibus,GEO)获取SLE和pSS基因表达数据集,进行加权基因共表达网络分析(weighted gene co-expression network analysis,WGCNA)和差异表达基因分析(differentially expressed genes,DEGs),初步确认SLE和pSS的共同关键基因和基因表达特征。结果WGCNA和DEGs分析结果都显示SLE和pSS的共同基因主要富集在I型干扰素(interferon,IFN)和病毒免疫相关的通路上(均有P<0.05);分析得到9个关键基因,分别是ISG15、IFIT3、OASL、OAS2、SERPING1、GBP1、DDX60、XAF1和DDX58,其中大部分是I型IFN相关基因。结论研究结果提示SLE和pSS的共同基因表达特征主要与I型IFN和病毒免疫相关基因以及通路有关,这为未来的机制研究提供了一定的参考。

Objective To initially investigate the common gene expression characteristic and molecular mechanism of systemic lupus erythematosus(SLE)and primary Sj9 gren’s syndrome(pSS).Methods The SLE and pSS gene expression datasets were obtained from the Gene Expression Omnibus(GEO)database.Weighted gene co-expression network analysis(WGCNA)and differentially expressed genes(DEGs)were performed on the data to preliminarily identify the common key genes and gene expression characteristics of SLE and pSS.Results Both WGCNA and DEGs analysis showed that the common genes of SLE and pSS were mainly enriched in type I interferon(IFN)and viral immune-related pathways(all P<0.05).The analysis identified nine key genes,namely ISG15,IFIT3,OASL,OAS2,SERPING1,GBP1,DDX60,XAF1,and DDX58,most of which were type I IFN-related genes.Conclusion The findings suggest that the common gene expression signatures of SLE and pSS are mainly related to type I IFN and viral immune-related genes and pathways,which will provide some reference for future mechanistic studies.