金线莲组培苗快速培养研究

Study on Rapid Propagation of Anoectochilus roxburghii Tissues Culture Seedling

通过筛选金线莲外植体诱导愈伤组织、不定芽诱导分化和无根苗壮苗生根的最佳培养基配方,建立金线莲组培苗快速繁育体系.结果表明,在5种外植体中,叶柄和茎两部分外植体能诱导出愈伤组织;叶柄诱导愈伤组织的最佳培养基配方为1/2 MS+NAA 0.6 mg/L+ZT 0.5 mg/L+6-BA 0.5 mg/L,培养50 d后统计愈伤组织诱导率为95.0%;不定芽诱导的最佳培养基为1/2 MS+ZT 0.4 mg/L+NAA 0.5 mg/L+6-BA 1.5 mg/L,培养30 d统计不定芽诱导率达93%;金线莲壮苗生根最佳培养基为1/2 MS+活性炭1 g/L+NAA 0.3 mg/L,培养40 d统计生根率为100%,且组培苗长势健壮;将组培苗移至由草炭泥和锯末(1∶1)组成的基质中,在温度(20±2)℃,相对湿度在90%左右和光照强度为400 lx的条件下培养25 d后统计金线莲苗的存活率为88%.

The paper is going to establish a rapid propagation system of tissue culture seedling of Anoectochilus roxburghii. Callus induction from explants,adventitious buds induction and differentiation and root culture of rootless seedlings were screened to find the best medium formula. The results showed that among five explants only petiole and stem explants could induce callus,and the optimal medium formula was 1/2 MS + NAA 0. 6 mg/L + ZT 0. 5 mg/L + 6-BA 0. 5mg/L. After 50 days of culture,the callus induction rate was 95. 0 %. The optimal medium for adventitious bud induction was 1/2 MS + ZT 0. 4 mg/L +NAA 0. 5 mg/L + 6-BA 1. 5 mg/L,and the rate of adventitious bud induction was 93% after 30 days of culture. The optimal rooting medium was 1/2 MS + activated carbon 1 g/L + NAA 0. 3 mg/L,and the rooting rate was 100% after 40 days of culture. The tissue culture seedlings were transferred to the substrate composed of peat mud and sawdust( 1 ∶ 1),and cultured at( 20 ± 2) ℃,90% relative humidity and 400 lx light intensity for 25 days. The survival rate of the seedlings was 88%.