蛇足石杉内生真菌Cladosporium tenuissimum MT-35的代谢产物研究

Metabolites of endophytic fungus Cladosporium tenuissimum MT-35 from Huperia serrata

为研究蛇足石杉内生真菌极细枝孢菌(Cladosporium tenuissimum MT-35)的代谢产物,本实验采用硅胶柱色谱及半制备液相色谱等方法从蛇足石杉极细枝孢菌C.tenuissimum MT-35的糙米发酵物中分离出10个化合物,根据化合物的理化性质及MS、NMR数据分别鉴定为4-hydroxy-3-methoxy-2(1 H)-quinolinone(1)、acropyrone(2)、lumichrome(3)、(1 H-indol-3-yl)oxoacetamide(4)、1,6-dihydro-1-methyl-6-oxo-3-pyridinecarboxylic acid(5)、尿嘧啶(6)、过氧化麦角甾醇(7)、麦角固醇(8)、9(Z),12(Z)-十八碳二烯酰胺(9)、8-O-4-dehydrodiferulic acid(10)。除化合物7和8外,其他化合物均为首次从C.tenuissimum中分离得到。对分离鉴定的化合物进行体外抗炎和抗乙酰胆碱酯酶活性筛选,结果表明化合物3、7、8、9对脂多糖诱导的小鼠单核巨噬细胞释放一氧化氮具有抑制活性,IC_(50)值分别为36.8±1.0、13.9±0.2、36.6±0.0、53.2±1.4μmol/L,其余化合物在浓度为100μmol/L时,对RAW 264.7细胞NO释放无明显抑制活性;化合物3对乙酰胆碱酯酶具有一定的抑制活性,IC_(50)为77.9±11.1μmol/L,其余化合物在浓度为100μmol/L时,对乙酰胆碱酯酶无明显抑制活性.

To study the metabolites of Cladosporium tenuissimum MT-35,an endophytic fungus isolated from Huperzia serrata.The compounds were separated and purified by silica gel column chromatography and semi-preparative HPLC.Ten compounds were isolated from the brown rice fermentation of the endophytic fungus C.tenuissimum MT-35,including 4-hydroxy-3-methoxy-2(1 H)-quinolinone(1),acropyrone(2),lumichrome(3),(1 H-indol-3-yl)oxoacetamide(4),1,6-dihydro-1-methyl-6-oxo-3-pyridinecarboxylic acid(5),uracil(6),ergosterol peroxide(7),ergosterol(8),9(Z),12(Z)-octadecadienamide(9),8-O-4-dehydrodiferulic acid(10).Except for compounds 7 and 8,other compounds were obtained from C.tenuissimum for the first time.Compounds 3,7,8 and 9 showed inhibitory activity against NO release from RAW 264.7 cells with IC_(50) values of 36.8±1.0,13.9±0.2,36.6±0.0,53.2±1.4μmol/L,respectively.Compound 3 showed inhibitory activity against acetylcholinesterase with an IC_(50) value of 77.9±11.1μmol/L.