趋化因子CXCR7/CXCL11/CXCL12生物轴在狼疮肾炎患者外周血中表达研究

Expression of CXCR7, CXCL11 and CXCL12 biological axis in the peripheral blood of patients with lupus nephritis

目的 探讨CXC型趋化因子受体7(CXCR7)与双配体CXC型趋化因子配体(CXCL)11、CXCL12在狼疮肾炎(LN)外周血中表达水平及意义。方法 选取江苏省徐州市中心医院(以下简称“我院”)风湿免疫科2018年10月至2020年10月就诊的40例系统性红斑狼疮(SLE)患者为研究对象。SLE患者分为LN组(24例),非LN组(16例)。20名健康对照组为我院同期体检者。采用流式细胞术分别检测三组外周血中CD3;CXCR7;T淋巴细胞、CD19;CXCR7;B淋巴细胞表达。采用酶联免疫吸附试验法检测各组血清中CXCL11、CXCL12浓度水平。结果 LN组外周血中CD3;CXCR7;T淋巴细胞、CD19;CXCR7;B淋巴细胞表达百分比及血清中CXCL11、CXCL12表达水平均高于非LN组和健康对照组,差异均有统计学意义(P <0.05)。非LN组CD3;CXCR7;T淋巴细胞、CD19;CXCR7;B淋巴细胞、CXCL11、CXCL12表达水平高于健康对照组,差异均有统计学意义(P <0.05)。LN患者外周血中CD19;CXCR7;淋巴细胞、CD3;CXCR7;淋巴细胞、CXCL11、CXCL12表达均与24 h尿蛋白定量呈正相关(P <0.001)。结论 LN患者发病与CXCR7、CXCL11、CXCL12高表达有关,提示CXCR7/CXCL11/CXCL12生物轴可能在狼疮肾炎的发病中起重要作用。

Objective To investigate the expression level and significance of CXC chemokine receptor 7(CXCR7) and CXC-type chemokine ligand(CXCL) 11 and CXCL12 in peripheral blood of lupus nephritis(LN). Methods Forty patients with systemic lupus erythematosus(SLE) treated in the Department of Rheumatology, Xuzhou Central Hospital(“our hospital” for short) from October 2018 to October 2020 were selected as the study subjects. SLE patients were divided into the LN group(n 24 cases) and the non-LN group(16 cases). Twenty healthy subjects in the control group were examined in our hospital during the same period. Flow cytometry was used to detect the expression of CD3;CXCR7;T lymphocytes and CD19;CXCR7;B lymphocytes in peripheral blood of the three groups. The concentrations of CXCL11 and CXCL12 in serum were detected by enzyme-linked immunosorbent assay. Results The expression percentage of CD3;CXCR7;T lymphocyte and CD19;CXCR7;B lymphocyte in peripheral blood and the expression levels of CXCL11 and CXCL12 in serum of LN group were higher than those of non-LN group and healthy control group, the differences were statistically significant(P < 0.05). The expression levels of CD3;CXCR7;T lymphocytes, CD19;CXCR7;B lymphocytes, CXCL11 and CXCL12 in the non-LN group were higher than those of healthy control group, the differences were statistically significant(P < 0.05). The expression levels of CD19;CXCR7;lymphocytes, CD3;CXCR7;lymphocytes, CXCL11, and CXCL12 in peripheral blood of patients with LN were positively correlated with the quantification of 24 h urinary protein(P < 0.001). Conclusion The high expression levels of CXCR7, CXCL11, and CXCL12 are related to the pathogenesis of LN patients.The CXCR7/CXCL11/CXCL12 may play an important role in the pathogenesis of LN.