摘要
为探究湘西地区吕氏泰勒虫的单倍型多样性及其种系发育关系,以采自于湘西地区的16株羊源吕氏泰勒虫为研究对象,PCR扩增其核糖体18S rRNA、线粒体细胞色素c氧化酶亚基Ⅰ(cox1)基因序列,并分析其单倍型多态性及种系发育关系。结果表明,湘西地区吕氏泰勒虫核糖体18S rRNA基因序列的长度为384 bp,含9个变异位点、7个单倍型(A1—A7),单倍型多样性Hd为0.775;cox1基因序列的长度为1 092 bp,存在2个变异位点、4个单倍型(B1—B4),单倍型多样性Hd为0.625;在18S rRNA+cox1重组的基因序列中,共含有11个变异位点、10个单倍型(C1—C10),单倍型多样性Hd为0.925;其中,A1、A4、B1、B3、B4、C1、C8与C9均为古老单倍型。系统发育分析结果显示,供试的16株羊源吕氏泰勒虫均处于系统发育树的同一分支上。这说明来自于湘西地区的16株羊源吕氏泰勒虫之间尽管存在一定程度的基因变异现象和单倍型多样性,但它们之间存在共同古老的单倍型,表明其个体之间和单倍型之间均具有较近的亲缘关系,未出现遗传分化。
The aim of the study was to explore the haplotype diversity and phylogenetic relationships of Theileria luwenshuni(T. luwenshun)from goats in western Hunan. A total of 16 strains of T. luwenshun from goats in western Hunan were used as study samples,and the polymerase chain reaction was applied to amplify the ribosomal 18S rRNA and cytochrome c oxidase subunit 1(cox1)genes of T. luwenshun to study their haplotype diversity and phylogenetic relationships. Results of our study showed that all the sequences of 18S rRNA and cox1 genes of T. luwenshun were 384 bp and 1 092 bp,respectively. The 18S rRNA and cox1 gene sequences contained 9 and 2 variable sites,respectively,and included 7(A1—A7,Hd=0. 775)and 4(B1—B4,Hd=0. 625)haplotypes,respectively;there were 11 variable sites and 10(C1—C10,Hd=0. 925) haplotypes in the combined sequences of 18S rRNA + cox1. Out of these haplotypes,the A1,A4,B1,B3,B4,C1,C8 and C9 were ancient haplotypes. In addition,all the 16strains of T. luwenshun from goats were located at a clade of phylogenetic tree constructed by using18S rRNA + cox1 gene sequences. The results of our study show that although there is a certain degree of gene variation among 16 strains of T. luwenshuni from western Hunan,they share several ancient haplotypes and have a close genetic relationships,and there is no obvious genetic differentiation.
作者
李中波
侯强红
李晖
舒鸣
LI Zhongbo;HOU Qianghong;LI Hui;SHU Ming(Huaihua Vocational and Technical College,Huaihua 418000,China;Xiangxi Vocational and Technical College,Jishou 416000,China)
出处
《河南农业科学》
北大核心
2022年第4期130-137,共8页
Journal of Henan Agricultural Sciences
基金
湖南省教育厅项目(20C1498)
湖南省怀化市科学技术局项目(2020R3107)。