复方卡森糖浆缓解D-氨基半乳糖引起的小鼠急性化学性肝损伤

Compound Casson Syrup Alleviates Acute Chemical Liver Injury in Mice Caused by D-galactosamine

目的探讨复方卡森糖浆对D-氨基半乳糖(D-galactose)致小鼠急性化学性肝损伤的保护作用。方法通过腹腔注射D-氨基半乳糖800 mg/kg建立小鼠化学性肝损伤模型。根据服用药物及剂量不同分为模型对照组(不服用药物),复方卡森糖浆低剂量组、中剂量组、高剂量组,炎消迪娜儿糖浆阳性对照组,同时设立正常对照组。每组8只。复方卡森糖浆(8 mL·kg^(-1)·d^(-1))与炎消迪娜儿糖浆(12 mL·kg^(-1)·d^(-1))分别灌胃后,检测小鼠血清生化、肝功能及肝损伤免疫学指标的变化,并通过HE染色观察肝组织病理性改变。结果与正常对照组相比,模型组小鼠肝脏指数升高(P<0.05),提示肝损伤。与模型组比较,复方卡森糖浆治疗后血清生化、肝功能指标均有所下降(P<0.05)。与对照组比较,模型组小鼠肝脏匀浆中丙二醛(malondialdehyde,MDA)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性增高(P<0.05),超氧化物歧化酶(superoxide dismutase,SOD)活性明显降低(P<0.01)。与模型组比较,复方卡森糖浆各剂量组小鼠SOD活性均升高(P<0.05)。HE染色结果显示,复方卡森糖浆组的小鼠肝脏炎性细胞浸润、变性、坏死等肝损伤病理现象有所改善。结论复方卡森糖浆能有效缓解D-氨基半乳糖诱导的小鼠肝损伤状态,对肝脏有一定的保护作用。

Objective To investigate the protective effect of compound Casson syrup on acute chemical liver injury induced by D-galactose in mice.Methods The chemical liver injury model was established by intraperitoneal injection of D-galactose 800 mg/kg.The experiment was divided into 6 groups,including normal control group,model control group,positive control group of Yanxiaodinar syrup,and compound Casson syrup low-dose,medium-dose and high-dose groups,with 8 rats in each group.After intragastric administration of compound Casson syrup(8 mL·kg^(-1)·d^(-1))and Yanchaudinar syrup(12 mL·kg^(-1)·d^(-1)),the changes of serum biochemistry,liver function and immunological indexes of liver injury were detected,and the pathological changes of liver tissue were observed.Results Compared with the normal control group,the liver index of model group was increased(P<0.05),suggesting liver injury.Compared with model group,serum biochemical and liver function indexes were decreased after treatment with compound Casson syrup(P<0.05).Compared with normal control group,the activities of malondialdehyde(MDA)and glutathione peroxidase(GSH-Px)in liver homogenate of model group were increased(P<0.05),and the activity of superoxide dismutase(SOD)was significantly decreased(P<0.01).Compared with model group,SOD activity in compound Casson syrup groups was increased(P<0.05).HE staining showed the inflammatory cell infiltration,degeneration,necrosis and other pathological phenomena of mouse liver caused by D-galactose were reversed after Casson syrup treatment.Conclusion Compound Casson syrup can effectively relieve the liver injury induced by D-galactose,and has a certain protective effect on liver.