甜菜SBPase基因的遗传转化

Genetic Transformation of Sugar Beet with SBPase Gene

利用转基因方法在甜菜中适度表达光合作用相关的关键酶(景天庚酮糖-1,7-二磷酸酶,SBPase),具有提高光合效率,提高甜菜产量的潜力。本研究以甜菜叶柄为受体材料,构建携带SBPase基因的植物表达载体,通过农杆菌介导法进行遗传转化;利用RT-PCR技术检测转基因植株中SBPase基因的表达情况;通过微滴数字PCR技术筛选低拷贝转基因植株。在所检测的抗性植株中获得转录水平上表达的SBPase转基因甜菜植株8株,其中低拷贝株系2株,为探索甜菜产量形成机理,创制高产新种质奠定了基础。

The use of transgenic methods to moderately express key enzymes related to photosynthesis(Sedum heptulose-1,7-bisphosphatase,SBPase)in sugar beet has the potential to improve photosynthetic efficiency and increase sugar beet yield.In this study,beet petiole was used as receptor material,and the plant expression vector carrying SBPase gene was used for genetic transformation by Agrobacterium-mediated method;RT-PCR technology was used to detect the expression of SBPase in transgenic plants;Low-copy transgenic plants were screened by droplet digital PCR technology.We obtained 8 transgenic sugarbeet plants with detected SBPase gene expressing at the transcriptional level,including 2 low-copy lines,which laid the foundation for the research on exploring the formation mechanism of sugar beet yield and creating new germplasm with high yield potential.