摘要
目的建立丹参麦味颗粒的质量标准。方法采用薄层色谱法(TLC)对丹参麦味颗粒中丹参、赤芍、佛手、芦根、麦冬、当归、川芎、酒女贞子、制何首乌进行定性鉴别,采用高效液相色谱法(HPLC)对丹酚酸B进行含量测定。色谱柱:Shim-pack VP-ODS柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸水(22∶78),检测波长286 nm,流速1.0 mL/min,柱温30℃。结果TLC鉴别图谱显示:主斑点清晰,分离度好且阴性无干扰;丹酚酸B 0.1728~0.6048μg范围内线性关系良好(r^(2)=0.9993),低浓度、中浓度、高浓度的平均加样回收率分别为95.58%、96.60%、98.19%,相对标准偏差(RSD)为1.50%。结论建立的鉴别和含量测定方法专属性强、准确度高、可重复性和稳定性好,为有效控制丹参麦味颗粒的质量控制提供参考和数据支持。
Objective To establish the quality standard for Danshen Maiwei Granules.Methods Qualitative identification of Salviae Miltiorrhizae Radix et Rhizoma(Danshen),Paeoniae Radix Rubra(Chishao),Citri Sarcodactylis Fructus(Foshou),Phragmitis Rhizoma(Lugen),Ophiopogonis Radix(Maidong),Angelicae Sinensis Radix(Danggui),Chuanxiong Rhizoma(Chuanxiong),and wine-fried Ligustri Lucidi Fructus(Jiunvzhenzi)and Polygoni Multiflori Radix Praeparata(zhiheshouwu)in Danshen Maiwei Granules by thin-layer chromatography(TLC).Determination of salvianolic acid B were tested by high performance liquid chromatography(HPLC).With chromatographic column:shim-pack VP-ODS column(250 mm×4.6 mm,5μm),mobile phase:acetonitrile-0.1%phosphoric acid water(22∶78),detection wavelength 286 nm,flow rate 1.0 mL/min,column temperature 30℃.Results TLC identification map showed:the main spot was clear,the separation was better without negative interference,salvianolic acid B 0.1728~0.6048μg showed a good linear relationship in the concentration range(r^(2)=0.9993),and the average sample recovery rates of low concentration,medium concentration and high concentration were 95.58%,96.60%and 98.19%,respectively.The relative standard deviation(RSD)was 1.50%.Conclusion The established identification and content determination method might be a method with strong specificity,high accuracy,good repeatability and stability,which could provide reference and data support for the effective quality control of Danshen Maiwei Granules.
作者
王婷
江欢欢
刘恩荔
房嘉程
张思蔚
扈明玉
乔丽俊
郑晓俊
WANG Ting;JIANG Huanhuan;LIU Enli;FANG Jiacheng;ZHANG Siwei;HU Mingyu;QIAO Lijun;ZHENG Xiaojun(Shanxi Medical University,Taiyuan 030001,Shanxi,China)
出处
《中西医结合心脑血管病杂志》
2022年第12期2184-2190,共7页
Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
关键词
丹参麦味颗粒
质量标准
丹酚酸B
薄层色谱法
高效液相色谱法
实验研究
Danshen Maiwei Granules
quality standard
salvianolic acid B
thin-layer chromatography
high performance liquid chromatography
experimental study