大豆7S与11S球蛋白QTL元分析及候选基因挖掘

Meta-analysis and candidate gene mining of soybean 7S and 11S globulin QTL

大豆蛋白作为重要营养来源,其含量在大豆种子中约占40%。7S球蛋白(b-伴大豆球蛋白)和11S球蛋白(大豆球蛋白)约占种子总蛋白70%,其含量直接影响大豆蛋白品质及特性。因此,大豆7S与11S球蛋白含量相关QTL定位与候选基因挖掘非常重要。研究调查国内外已报道的42个7S与11S球蛋白相关QTL数据,通过利用BioMercator ver.2.1软件对其进行元分析得到11个Meta-QTL区间。利用《京都基因与基因组百科全书》和基因本体数据库分析Meta-QTL区域基因,确定18个参与调控7S与11S球蛋白含量的相关基因作为候选基因,该候选基因分别参与植物生长发育、氨基酸生物合成等过程。其中1个基因被注释为编码2S白蛋白超家族蛋白,6个基因与蛋白质合成相关细胞器的生长发育相关,5个基因被注释为编码影响贮藏蛋白积累的转录因子家族蛋白,6个基因与贮藏蛋白运输的分子机制相关。研究结果对大豆7S和11S球蛋白相关QTL定位及分子辅助育种具有重要意义。

As an important source of nutrition,soybean protein accounts for about 40%of soybean seeds.Among them,7S globulin(b-conglycinin)and 11S globulin(glycinin)account for about70%of the total seed protein,and their contents directly affect the qualities and characteristics of soybean protein.Therefore,the mapping of soybean 7S and 11S globulin content-related QTLs and the mining of candidate genes are extremely important.The research investigated 42 QTL data related to7S and 11S globulin reported at home and abroad,and obtained 11 Meta-QTL intervals by metaanalysis using BioMercator ver.2.1 software.Using the Kyoto Encyclopedia of Genes and Genomes and the Gene Ontology database to analyze the genes in the Meta-QTL region,18 related genes involved in the regulation of 7S and 11S globulin content were identified as candidate genes.These candidate genes were involved in plant growth and development,amino acid biosynthesis and other processes.Among them,one gene was annotated as encoding 2S albumin superfamily protein,six genes were associated with the growth and development of protein synthesis-related organelles,five genes were annotated as encoding transcription factor family proteins affecting the accumulation of storage proteins,six genes were associated with storage Molecular mechanisms of protein transport.The experimental methods and results of this study were of great significance for the mapping of soybean7S globulin and 11S globulin related QTL and molecular-assisted breeding research.