二苯乙烯苷对糖尿病小鼠认知障碍的影响

Effects of Tetrahydroxy Stilbene Glycosides on Cognitive Impairment in Diabetic Mice

目的探讨二苯乙烯苷(TSG)对糖尿病(DM)小鼠认知障碍的改善作用及其机制。方法C57BL/6J小鼠高脂饲料喂养联合链脲佐菌素(STZ)40mg/kg腹腔注射制备糖尿病模型。将造模成功的DM小鼠随机分为DM模型组、TSG40mg/kg组、TSG80mg/kg组,每组10只;取正常饲料喂养的C57BL/6J小鼠10只作为正常对照组(Con组)。TSG连续灌胃8周后用Morris水迷宫检测各组小鼠认知功能,用生化试剂盒测定小鼠血浆和海马组织SOD、GSH-Px的活性及MDA、GSH含量。结果DM小鼠定位航行的逃避潜伏期明显延长,空间探索实验的目标象限滞留时间百分比明显缩短,海马组织和血浆中的SOD和GSH-PX活性以及GSH含量显著降低,MDA含量显著增高;40mg/kg和80mg/kg的TSG均能明显缩短DM小鼠定位航行潜伏期,延长空间探索实验的目标象限滞留时间百分比,增加海马组织和血浆中的SOD和GSH-PX活性以及GSH含量,减少MDA含量。结论TSG可改善糖尿病小鼠认知功能障碍,其机制可能与其抑制糖尿病诱导的氧化应激损伤有关。

Objective To explore the improvement effect and mechanism of tetrahydroxy stilbene glycoside(TSG)on cognitive impairment in diabetic(DM)mice.Methods C57BL/6J mice were fed with high-fat diet combined with streptozotocin(STZ)40mg/kg intraperitoneally to establish the diabetes model.The successfully established DM mice were randomly divided into DM model group,TSG 40mg/kg group,TSG 80mg/kg group,10 mice in each group;10 C57BL/6J mice fed with normal diet were taken as normal control group(ConGroup).After continuous gavage of TSG for 8 weeks,Morris water maze was used to detect the cognitive function of mice in each group,and biochemical kits were used to determine the activities of SOD and GSH-Px and the contents of MDA and GSH in the plasma and hippocampus of mice.Results The escape latency of positioning navigation in DM mice was significantly prolonged,the percentage of residence time in the targat quadrant of space exploration experiment was significantly shortened,the activities of SOD and GSH-PX in hippocampus and plasma were significantly decreased,the content of MDA was significantly increased,and the content of GSH was significantly decreased;TSG 40mg/kg and 80mg/kg can significantly shorten the latency of positioning and navigation of DM mice,prolong the escape platform retention time of DM mice in space exploration experiments,and increase the activities of SOD and GSH-PX and the content of GSH in hippocampus and plasma of DM mice.Conclusion TSG can improve cognitive dysfunction in diabetic mice,and its mechanism may be related to its inhibition of diabetes-induced oxidative stress damage.