微小RNA-486-5p在子宫内膜癌诊治中的临床价值研究

Clinical value of miR-486-5p in the diagnosis and treatment of endometrial cancer

目的探讨微小RNA-486-5p(miR-486-5p)在子宫内膜癌中的表达,并分析其与患者临床病理特征的关系。方法选取2013年1月至2017年6月唐山市妇幼保健院收治的88例行手术治疗的子宫内膜癌患者作为研究对象(子宫内膜癌组),收集手术切除的子宫内膜癌组织以及配对的癌旁组织。另选取同期收治的72例因子宫肌瘤(子宫内膜正常)行手术治疗的患者(子宫内膜正常组)。比较miR-486-5p表达水平,采用受试者工作特征(ROC)曲线评估血清miR-486-5p水平对子宫内膜癌的诊断价值;Kaplan-Meier法分析子宫内膜癌组织miR-486-5p表达与患者生存率的关系;多因素Cox回归分析影响子宫内膜癌预后的因素。结果与癌旁组织比较,子宫内膜癌组织中miR-486-5p表达水平较低(P<0.05);与子宫内膜正常组比较,子宫内膜癌组血清中miR-486-5p表达水平较低(P<0.05);血清中miR-486-5p水平预测子宫内膜癌的曲线下面积为0.872,灵敏度为79.20%,特异度为84.10%。miR-486-5p与浸润深度、组织类型相关(P<0.05)。结论在子宫内膜癌组织中miR-486-5p表达下降,与浸润深度、组织类型密切相关,可能成为新的子宫内膜癌治疗靶点。

Objective To investigate the expression of microRNA-486-5 p(miR-486-5 p)in endometrial cancer and its relationship with the clinical pathological characteristics of patients.Methods 88 patients with endometrial cancer who underwent surgical treatment in Tangshan Maternal and Child Health Hospital from January 2013 to June 2017 were selected as the research objects(endometrial cancer group),the surgically resected endometrial cancer tissues and matched adjacent tissue specimens were collected.Another 72 patients with uterine fibroids(normal endometrial group)who underwent surgical treatment during the same period were selected.The expression levels of miR-486-5 p were compared.The receiver operating characteristic(ROC)curve was used to evaluate the diagnostic value of serum miR-486-5 p levels in endometrial cancer.The Kaplan-Meier method was used to analyze the relationship between the expression of miR-486-5 p in endometrial cancer tissue and the survival rate of patients.Multivariate Cox regression was used to analyze the prognostic factors of endometrial cancer.Results Compared with adjacent tissues,the relative expression level of miR-486-5 p in cancer tissues was lower(P<0.05).Compared with the normal endometrial group,the relative expression level of miR-486-5 p in the serum of the endometrial cancer group was lower(P<0.05).The area under the curve(AUC)of serum miR-486-5 p level predicting endometrial cancer was 0.872,and its sensitivity and specificity were 79.20%and 84.10%,respectively.MiR-486-5 p was related to the depth of invasion and tissue type(P<0.05).Conclusions The expression of miR-486-5 p in endometrial cancer tissues is decreased,which is closely related to the depth of invasion and tissue type,and may become a new therapeutic target for endometrial cancer.