浙江樟3种组织及不同区段木材挥发性组分差异分析

Analysis of Volatile Components in Three Tissues and Timber of different Sections of Cinnamomum chekiangense

为探究浙江樟Cinnamomum chekiangense组织部位间、单株间和木材不同区段间挥发性成分的差异,提取和鉴定浙江樟叶片、干皮与木质部3种组织部位中的挥发性成分,以代表性原则采集样品,利用固相微萃取法-气相色谱-质谱联用法(SPME-GC-MS)结合计算机自动检索辅助人工解析挥发性成分,通过峰面积归一法测定其相对含量。从7年生浙江樟3种组织部位中共检测出主要挥发性成分134种。其中,干皮83种,独有成分40种;叶片77种,独有38种;木质部45种,9种是独有的。成分种类分析知,浙江樟不同组织部位挥发性成分包括烯烃类、烷烃类、醇类和酯类等9种类别;相对含量分析知,浙江樟各组织部位挥发性成分均以烯烃类化合物为主,组分数量和相对含量均远高于其它8类成分。通过进一步分析知,7年生浙江樟单株间挥发性成分有明显差异,24年生浙江樟木材的实际生长时间对木质部挥发性有机物的种类和相对含量也有明显影响。综合表明浙江樟不同组织部位间、单株间、不同区段木材间挥发性成分种类与组成特征异同明显,主要功能性组分与相对含量均存在一定差异。

In order to explore the differences of volatile components among tissues,individual plants and different sections of timber,the volatile components in leaves,bark and xylem of Cinnamomum chekiangense were extracted and identified.Samples were collected according to the representative principle,and the volatile components were analyzed through the solid phase microextraction-gas chromatography-mass spectrometry combined with computer automatic search assisted manual analysis.The relative content was determined by peak area normalization method.A total of 134 main volatile components were detected in 3 tissues of 7-year-old C.chekiangense.Among them,there were 83 species of bark and 40 unique components.Leaves were contained 77 substances including 38 unique substances.Xylem was contained 45 componets 9 unique components.According to the analysis of component types,the volatile components include 9 categories such as olefins,alkanes,alcohols and esters.Relative content analysis showed that olefins were the main volatile components,and the number and relative content of components were remarkable higher than the others.Through further analysis,it was found that there were significant differences in the volatile components among individuals of 7-year-old C.chekiangense,and the actual growth time of 24-year-old C.chekiangene timber also had a distinct influence on the species and relative content of volatile organic compounds in xylem.There were obvious similarities and differences in the types and composition of volatile components among different tissues,individual plants,and sections of the timber.Main functional components and relative contents were evidently different.