CNTN1基因在乳腺癌中的表达及其与预后关系的研究

Expression of contactin-1 in breast cancer tissues and its relation with prognosis of patients with breast cancer

目的检测接触蛋白-1(contactin-1,CNTN1)基因在乳腺癌组织中的表达及其与乳腺癌患者临床病理因素和预后的关系。方法回顾性收集陕西省肿瘤医院2015年1月至2015年6月期间符合纳入标准的35例乳腺癌患者的临床病理资料,采用免疫组织化学SP法和Western blot法检测乳腺癌组织及其相应的癌旁组织中CNTN1蛋白的表达情况,同时应用RT-PCR法检测CNTN1 mRNA在相应组织中的表达情况。分析乳腺癌组织中CNTN1蛋白表达与乳腺癌患者5年总生存情况的关系。结果乳腺癌组织中CNTN1蛋白表达阳性率和表达水平均高于其相应的癌旁组织[65.7%(23/35)比45.7%(16/35),χ^(2)=8.235,P=0.003;0.825±0.221比0.412±0.117,t=12.556,P<0.001],同时乳腺癌组织中CNTN1 mRNA表达水平亦高于其相应的癌旁组织(0.763±0.218比0.353±0.135,t=11.162,P<0.001)。CNTN1蛋白表达阳性率在肿瘤直径较大(>2 cm)、低分化、TNM分期较晚(Ⅲ+Ⅳ期)以及有腋窝淋巴结转移乳腺癌患者中更高(P<0.05)。35例乳腺癌患者的中位总生存期为47.3个月,其中CNTN1高表达(大于组织中CNTN1蛋白表达中位数0.785)患者的中位总生存期为36.2个月,低表达患者为52.5个月,CNTN1高表达患者的生存情况差于低表达患者(χ^(2)=4.052,P=0.049)。结论从本研究初步研究结果提示,CNTN1基因过表达可能在原发性乳腺癌的发生及进展中起重要作用且与生存预后有一定的关系。

Objective To detect the expression of contactin-1(CNTN1)gene in the breast cancer tissues and explore the relation between the expression and prognosis of patients with breast cancer.Methods The clinicopathologic data of 35 patients with breast cancer who met the inclusion criteria from January to June in 2015 in the Shaanxi Provincial Cancer Hospital were retrospectively collected.The Western blot and immunohistochemistry methods were used to detect the CNTN1 protein expressions in the 35 breast cancer tissues and their corresponding tissues adjacent to cancer and the reverse transcriptase-PCR method was used to detect the CNTN1 mRNA expression.The relation between the CNTN1 protein expression and 5-year overall survival(OS)was analyzed.Results The positive rate and expression level of CNTN1 protein in the breast cancer tissues were higher than those in the tissues adjacent to cancer[65.7%(23/35)vs.45.7%(16/35),χ~2=8.235,P=0.003;0.825±0.221 vs.0.412±0.117,t=12.556,P<0.001],and the expression level of CNTN1mRNA in the breast cancer tissue was also higher than that in the corresponding tissues adjacent to cancer(0.763±0.218vs.0.353±0.135,t=11.162,P<0.001).The positive rates of CNTN1 protein were higher in the patients with larger tumor diameter(>2 cm),lower differentiation,later TNM stage(stageⅢ+Ⅳ)and axillary lymph node metastasis(P<0.05).The median OS of 35 patients with breast cancer was 47.3 months,which was 36.2 months and 52.5 months in the patients with high CNTN1 expression and low CNTN1 expression(median expression of CNTN1 protein was 0.785 as critical value)respectively(χ~2=4.052,P=0.049).Conclusion Preliminary results of this study suggest that overexpression of CNTN1 gene might play an important role in occurrence and progression of primary breast cancer and is related to prognosis of survival.