不同固定液对组织化学冰冻切片效果的影响

Comparative Study on Ten Different Fixation Solutions Effects on Histochemistry Staining

本研究以实验动物的肝组织为材料选择10种不同的固定液,观察比较其对组织化学冰冻切片的影响,为组织化学实验做出高质量的冰冻切片提供实验基础。取实验动物的肝组织,冰冻切片后分别用10种固定液进行固定,然后进行糖组织化学染色,观察各组的细胞组织结构形态,细胞核、细胞质染色和核质对比度,以及结构清晰度等情况。结果表明,10种固定液都可用于固定肝组织化学冰冻切片,但通过镜下观察形态、染色效果、核浆对比度及结构清晰度各有差异。4%甲醛、4%多聚甲醛、4%中性甲醛等效果较好,配制简便,宜继续使用。AF固定液、Carnoy固定液,可酌情使用。AAF、AFA、EAF固定液及Bouin固定液,均可不再使用。甲醇效果较好,且无须配制、价格便宜、使用方便,宜普遍使用。

Ten different fixation solutions were used to animal liver tissue in order to reveal their effect on immunohistochemistry.Our study can provide valuable information to improve high quality experiment section.Animal liver tissue were fixed using ten different fixation solutions,and liver cell were stained by histochemistry methods.The morphology of liver cell were recorded by microscope.The results showed that all of ten different fixation solutions can be used to fix animal liver tissue.According to the results of liver cell morphology,4%formalin and 4%paraformalin and 4%neutral formalin have better effect,then the AF fixation solution and Carnoy fixation solution could be appropriately used,and AAF,AFA,EAF fixation、Bouin fixation solution should not be used.4%formalin is the best fixation solution,which can easily obtained and conveniently used.