lncRNA SH3BP5-AS1单核苷酸多态性与中国汉族人缺血性中风血瘀证显著相关

LncRNA SH3BP5-AS1 Polymorphisms Are Associated with Blood Stasis Syndrome of Ischemic Stroke in Chinese Han Population

目的研究旨在探讨中国汉族人群中lncRNA SH3BP5-AS1多态性rs11713836与缺血性中风(ischemic stroke,IS)遗传易感性及中医证候的关系。方法研究包括774例IS患者和793例对照组。采用Massarray SNP基因分型方法进行基因分型。采用《中风病中医辨证诊断标准》量表对IS患者进行中医证候鉴定。所有统计分析采用SPSS 17.0统计软件进行。结果在隐性模型下,lncRNA SH3BP5-AS1多态性rs11713836与缺血性中风血瘀证显著相关[OR(95%CI)=0.52(0.29-0.93),P=0.028],在校正了包括性别和年龄在内的协变量后,结果依然有统计学意义;而rs11713836与缺血性中风易感性及风、痰、火热、气虚证等中医证候易感性无相关性。此外,rs11713836与血清APO-B[隐性模型:OR(95%CI)=0.09(0.02-0.17),P=0.032]、VLDL[加性模型:OR(95%CI)=0.09(0.04-0.14),P<0.001;显性模型:OR(95%CI)=0.09(0.02-0.16),P=0.011;隐性模型:OR(95%CI)=0.19(0.09-0.30),P<0.001]、TG[加性模型:OR(95%CI)=0.15(0.05-0.24),P=0.003;显性模型:OR(95%CI)=0.14(0.01-0.27),P=0.041;隐性模型:OR(95%CI)=0.31(0.11-0.50),P=0.003]、FIB[显性模型:OR(95%CI)=0.20(0.04-0.35),P=0.012]显著相关,调整性别年龄后以上关联依然有统计学意义。结论lncRNA SH3BP5-AS1多态性rs11713836可能影响缺血性中风血瘀证的发生,有望作为缺血性中风血瘀证的生物标志物与潜在治疗靶点,并且可能影响缺血性中风患者的血脂代谢和凝血功能。

Objective The aim of the present study was to explore the association between the lncRNA SH3BP5-AS1 polymorphism rs11713836 and the genetic susceptibility of ischemic stroke(IS)and traditional Chinese medicine(TCM)syndromes of IS in Chinese Han population.Methods The study included 774 patients with IS and 793 control subjects.Genotyping was performed via the Massarray SNP genotyping method on Agena platform.The TCM Syndrome Differentiation Diagnostic Criteria for Apoplexy Scale was adopted to identify the TCM syndromes of patients with IS.Results The lncRNA SH3BP5-AS1 polymorphism was significantly associated with blood stasis syndrome under the recessive model(rs11713836:OR[95%CI]=0.52[0.29-0.93],P=0.028).The association remained after adjusting for sex and age under the recessive model(rs11713836:OR_(adj)[95%CI]=0.53[0.30-0.95],P_(adj)=0.034).In addition,rs11713836 was significantly associated with blood serum apolipoprotein B(APO-B),very low density lipoprotein(VLDL),triglyceride(TG)and fibrinogen(FIB)of patients with IS(all P_(adj)<0.05).Conclusions LncRNA SH3BP5-AS1 polymorphism rs11713836 may affect the occurrence of blood stasis syndrome in ischemic stroke,and is expected to serve as a biomarker and potential therapeutic target for blood stasis syndrome in ischemic stroke,and may affect lipid metabolism and coagulation function in patients with ischemic stroke.