福建三明汉族人群20个常染色体STR位点的遗传多态性研究

Genetic polymorphism of 20 autosomal STR loci in Han population from Sanming,Fujian Province

目的研究福建三明汉族人群20个常染色体STR基因座遗传多态性,评估其法医学中的应用价值。方法采用Microreader^(TM)21 Direct ID System试剂盒对三明汉族508例无关个体血样进行检测,获取20个基因座的数据资料,分析基因分布频率及群体遗传学参数。结果20个STR基因座的等位基因频率为(0.0010~0.5827),各基因座基因型分布符合Hardy-Weinberg平衡(P>0.05);多肽信息总量(PIC)为0.52~0.91;期望杂合度(He)为0.5965~0.9173;匹配率(PM)为0.0313~0.2339;个体识别能力(DP)为0.766~0.985;排除概率(PE)为0.287~0.831;累计个体识别能力(TDP)为1-1.1124×10^(-24)=0.999999999999999999999999;累计排除率(CPE)为1-2.4513×10^(-9)=0.999999998。结论20个常染色体STR基因座在三明汉族人群中呈高度多态性和较好的鉴别能力,对个体识别及亲权鉴定具有较大法医学应用价值。

Objective To investigate the genetic polymorphism of 20 autosomal short tandem repeat(STR)loci in Han population in Sanming,Fujian,and to evaluate their forensic application value.Methods The Microreader^(TM)21 Direct ID System was used to test the blood of 508 unrelated Han individuals from Sanming to acquire the data of 20 autosamal STR loci,and calculate allele frequencies and population genetics parameters.Results The frequency of 20 STR loci ranged from 0.0010 to 0.5827,the distribution of all loci genotyges was Hardy-Weinberg equilibration(P>0.05);Polymorphism information content(PIC)ranged from 0.52 to 0.91,Heterozygosity(He)ranged from 0.5965 to 0.9173,Matching probability(PM)ranged 0.0313 to 0.2339;Discrimination power(DP)ranged 0.766 to 0.985;Exclusion power(PE)ranged 0.287 to 0.831;Total Probability of Discrimination Power(TDP)was 1-1.1124×10^(-24)=0.999999999999999999999999;Cumulative probability of exclusion(CPE)was 1-2.4513×10^(-9)=0.999999998.Conclusion The 20 autosomal STR loci showed a high polymorphism and discriminating ability in Han population from Sanming,Fujian Province.These autosomal STR loci can be valuable in personal indentification and paternity test in Sanming Han population.