转录组测序分析ZNF185在食管鳞状细胞癌中的作用机制

Analysis of the Mechanisms of ZNF185 in Esophageal Squamous Cell Carcinoma Based on Transcriptome Sequencing

目的通过转录组测序及生物信息学技术分析ZNF185在食管鳞状细胞癌(食管鳞癌)中的作用机制及其下游信号通路。方法利用shRNA转染食管鳞癌细胞系Eca-109敲低ZNF185的表达,采用转录组测序技术检测mRNA的表达变化。根据测序数据筛选差异基因,通过GO功能分析和KEGG富集分析对差异基因相关的功能及信号通路进行分析。通过STRING工具分析差异蛋白的相互作用,构建出相关的蛋白互作网络,分析关键节点蛋白。结果ZNF185干扰组与干扰对照组Eca-109细胞的差异基因共有3859个,其中表达升高的基因有2112个,表达下降的基因有1747个。显著富集的GO项目包括RNA剪接、细胞-基质连接、局部粘附等。KEGG富集分析表明ZNF185可能与HIF-1信号通路、p53信号通路有关,蛋白互作网络分析发现了BMP2、OAS2等关键蛋白。结论通过转录组测序和生物信息分析筛选出ZNF185参与调控食管鳞癌可能的信号通路,为阐明食管鳞癌的发病机制提供了新的思路。

Objective To explore the mechanism and down stream signaling pathways of ZNF185 in esophageal squamous cell carcinoma by transcriptome sequencing and bioinformatics analysis.Methods The expression of ZNF185 was knocked down by conducting shRNA transfection of esophageal squamous carcinoma cell lines,and the changes of mRNA expression were detected by transcriptome sequencing technology.The differential genes were screened based on sequencing data,and the differential gene-related functions and signaling pathways were analyzed by GO functional analysis and KEGG enrichment analysis.The interactions of differential proteins were analyzed by STRING,and the related protein-protein interaction(PPI)network was constructed to analyze the key node proteins.Results There were 3859 differential genes between shZNF185 group and the control group,including 2112 genes with elevated expression and 1747 genes with decreased expression.RNA splicing cell-matrix junction,local adhesion were significantly enriched in GO analysis.KEGG enrichment analysis indicated that ZNF185 might be related to HIF-1 signaling pathway and p53 signaling pathway,and key proteins such as BMP2 and OAS2 were identified in PPI network.Conclusion The possible signaling pathways involved in the regulation of esophageal squamous carcinoma by ZNF185 were screened out by transcriptome sequencing and bioinformatic analysis,which provide a new idea to elucidate the pathogenesis associated with esophageal squamous carcinoma.