异叶青兰总黄酮对离体大鼠胸主动脉环的舒张作用及其机制

Study on the Effect and Mechanism of the Total Flavonoids of Dracocephalum Heterophyllum Benth on Thoracic Aortic Ring in Rats

探讨异叶青兰总黄酮(DHBF)对大鼠胸主动脉血管环张力的影响,并探讨其可能的作用机制,为新疆地方药材的开发和有效利用提供理论依据。采用离体血管灌流技术,通过生物信号采集和分析系统测定不同质量浓度的DHBF(0.015,0.030,0.060,0.120,0.240和0.360 mg/L)对大鼠胸主动脉环血管环张力的影响。结果显示:(1)不同质量浓度的DHBF对静息状态下血管环的张力无明显影响(P>0.05);(2)与对照组比较,DHBF对去甲肾上腺素预收缩的内皮完整组和去内皮组血管环均具有明显的舒张作用(P<0.05),且内皮完整组的舒张作用明显强于去内皮组(P<0.05);与对照组比较,DHBF对KCl预收缩的血管环有明显的舒张作用(P<0.05),且内皮完整组和去内皮组血管环的差异无统计学意义(P>0.05);(3)与对照组和去内皮组比较,DHBF+L-NAME组(DHBF+MB)组对血管环的舒张作用明显被抑制(P<0.05),并呈浓度依赖性。DHBF对NE及KCl预收缩的血管环有明显的舒张作用,这可能与抑制了胞内肌浆网钙释放和Ca^(2+)通道等有关,且部分依赖于内皮细胞,这可能与作用于内皮依赖性的NO-cGMP信号通路有关。

To study the effect of total flavonoids(DHBF)on the thoracic aorta and explore the possible mechanism to provide theoretical basis for the development and effective utilization of local medicinal materials in Xinjiang,the effect of DHBF(0.015,0.030,0.060,0.120,0.240 and 0.360 mg/L)in the rat thoracic aorta was determined by the biosignal collection and analysis system.The results showed(1)Different concentrations of DHBF has no effecct on vascular ring tension in the resting state(P>0.05);(2)Comparison with the controls,the DHBF had a significant diastolic effect on both the NE precontracted endothelial-intact and endothelial-free groups(P<0.05),the diastolic effect of the intact endothelial group was significantly stronger than that in the endothelial-free group(P<0.05).In comparison with the control group,the DHBF showed significant diastolic effects on all the KCl pre-contracted vascular rings(P<0.05),there was no significant difference in the endothelial intact and endothelial-free groups(P>0.05);(3)Comparison with the control and endothelium-free groups,the diastolic effect of the vascular ring of the DHBF+L-NAME(DHBF+MB)group was significantly inhibited(P<0.05),and it showed a concentration-dependence.DHBF has obvious diastolic effect on NE and KCl,this may be related to the inhibition of intracellular muscle cytoplasmic network calcium release and Ca^(2+)channels,and it is partly dependent on endothelial cells,this may be related to the NO-cGMP signaling pathway.