钙镁对渗滤液处理中微生物电子传递体系的影响

Effect of Calcium and Magnesium Ions on Microbial Electron Transport System in Landfill Leachate Treatment

垃圾渗滤液中普遍存在的高浓度钙、镁离子会对微生物生长代谢产生抑制作用,而电子传递体系在微生物代谢过程中发挥着重要作用,因此,采用直接均分射线法设计3种不同钙离子和镁离子配比(L_(1)、L_(2)、L_(3)),采用Logistic和BiPhasic型曲线对浓度-效应数据进行拟合,并绘制浓度-效应曲线,利用等效线图法分析钙、镁离子对垃圾渗滤液处理中厌氧微生物电子传递体系的影响。结果表明,钙离子单独作用及钙、镁离子混合作用对厌氧微生物电子传递体系活性均表现为低浓度促进、高浓度抑制的特点,促进作用随钙、镁离子浓度的增加而减小,而抑制作用则随钙、镁离子浓度及作用时间的增加而增大。等效线图模型分析表明,当钙、镁离子配比为L_(1)和L_(2)时,随着作用时间的增加,钙、镁离子对厌氧微生物电子传递体系的作用关系由拮抗作用过渡到部分加和作用,最终转变为协同作用;当钙、镁离子配比为L_(3)时,钙、镁离子对厌氧微生物电子传递体系的作用关系为协同作用。

The high concentration of calcium and magnesium ions in landfill leachate can inhibit the growth and metabolism of microorganisms,and the electron transport system plays an important role in the metabolic process of microorganisms.Therefore,three different calcium and magnesium ion ratios(L_(1),L_(2) and L_(3))were designed by direct uniform beam method.Logistic and BiPhasic curves were employed to fit the concentration-effect data and draw the concentration-effect curve.The effect of calcium and magnesium ions on the electron transport system of anaerobic microorganisms in landfill leachate treatment was analyzed by isobologram method.The effects of calcium ion alone and calcium and magnesium ions together on the activity of anaerobic microbial electron transport system were characterized by low concentration promoting and high concentration inhibiting.The promoting effect decreased with the increase of calcium and magnesium ion concentration,while the inhibiting effect increased with the increase of calcium and magnesium ion concentration and action time.The isobologram model analysis showed that,when the ratios of calcium and magnesium ions were L_(1) and L_(2),the effect of calcium and magnesium ions on the anaerobic microbial electron transport system was transformed from antagonism to partial adding effect and eventually to synergy with the increase of action time.When the ratio of calcium and magnesium ions was L_(3),the effect of calcium and magnesium ions on the electron transport system was synergistic.